Sökning: "affinity"

Visar resultat 11 - 15 av 1392 avhandlingar innehållade ordet affinity.

  1. 11. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Författare :Malin Mejàre; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER

  3. 12. Introducing weak affinity chromatography to drug discovery with focus on fragment screening

    Författare :Minh-Dao Duong-Thi; Sten Ohlson; Brian Dymock; Linnéuniversitetet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity LC-MS; fragment-based drug discovery; fragment screening; high throughput; mass spectrometry; stereoisomer; enantiomer; thrombin; weak affinity chromatography; WAC; WAC-MS.; Biomedical Sciences; Biomedicinsk vetenskap; Biokemi; Biochemistry;

    Sammanfattning : Fragment-based drug discovery is an emerging process that has gained popularity in recent years. The process starts from small molecules called fragments. One major step in fragment-based drug discovery is fragment screening, which is a strategy to screen libraries of small molecules to find hits. LÄS MER

  4. 13. Isolation of caveolae using affinity two-phase partitioning

    Författare :Parisa Abedinpour; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; basolateral; apical; immunoaffinity; wheat germ agglutinin; caveolin; caveolae caveolae enriched fraction; plasma membranes; rat lung; rat liver; Affinity Partitioning; two-phase partitioning;

    Sammanfattning : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. LÄS MER

  5. 14. Investigations of chemical and enzymatic functionalization of affinity proteins

    Författare :Anders Myrhammar; Amelie Eriksson Karlström; Per-Åke Nygren; Christian Heinis; KTH; []
    Nyckelord :Affinity protein; affibody; conjugation; solid phase peptide synthesis; proteolytic stability; peptide nucleic acid PNA .; Bioteknologi; Biotechnology;

    Sammanfattning : AbstractAffinity proteins are important reagents in research, diagnostics and therapeutic settings. The focus of this thesis has been on investigating different chemical and enzymatic strategies for engineering of affinity proteins to generate affinity reagents with improved or changed functionality. LÄS MER

  7. 15. On the application and validation of multiplexed affinity assays

    Författare :Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Bioteknologi; Biotechnology;

    Sammanfattning : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. LÄS MER