Sökning: "wheat germ agglutinin"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden wheat germ agglutinin.

  1. 1. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes

    Författare :Ingo Gottschalk; Irving W. Wainer; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Affinity; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dissociation constant; Drug absorption; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Solute; Specific; Streptavidin; Wheat germ agglutinin; Biokemi; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. LÄS MER

  3. 2. Isolation of caveolae using affinity two-phase partitioning

    Författare :Parisa Abedinpour; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; basolateral; apical; immunoaffinity; wheat germ agglutinin; caveolin; caveolae caveolae enriched fraction; plasma membranes; rat lung; rat liver; Affinity Partitioning; two-phase partitioning;

    Sammanfattning : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. LÄS MER

  4. 3. Cervicothalamic tract termination and its cortical connections: anatomical studies in cats and ferrets

    Författare :Mengliang Zhang; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neuropsychology; Neurology; Carnivora; Cytoarchitecture; Immunocytochemistry; Compartmentation; Retrograde transport; Anterograde transport; Somatosensory cortex; Thalamus; Spinal cord; Lateral cervical nucleus; neurophysiology; Neurologi; neuropsykologi; neurofysiologi;

    Sammanfattning : The termination and cortical connections of the cervicothalamic tract (CTT) were studied by neuroanatomical tract tracing techniques in cats and ferrets. After injections of wheat germ agglutinin-horseradish peroxidase conjugate or biotinylated dextran amine (BDA) into the cat lateral cervical nucleus (LCN), the most abundant CTT termination is present in the ventral posterior lateral nucleus (VPL). LÄS MER

  5. 4. Central projections of sensory innervation of cranial vessels

    Författare :Yi Liu; Lund Medicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurologi; Spinothalamic; Neurology; neuropsychology; neurophysiology; Neural tracing; trigeminal nerve; Headache; Cranial vessel; Brainstem; neuropsykologi; neurofysiologi;

    Sammanfattning : Primary headaches (e.g. migraine and cluster headache), the symptoms of which is associated with cranial vascular activity, is likely to be dependent on a primary central nervous system dysfunction. LÄS MER

  7. 5. Phosphatidylinositol 4-kinases in plasma membranes and Affinity partitioning of liposomes: a model system for membrane purification

    Författare :Lars Ekblad; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; metabolism; Biokemi;

    Sammanfattning : Polyphosphoinositides, the different phosphorylated variants of the phospholipid phosphatidylinositol (PtdIns), are used in a wide array of intracellular mechanisms in eukaryotic cells. The phosphorylation of PtdIns at carbon D4, catalyzed by phosphatidylinositol 4-kinase, is an important step in the synthesis of several of these molecules. LÄS MER