Sökning: "Biokemi"

Visar resultat 1 - 5 av 1849 avhandlingar innehållade ordet Biokemi.

  1. 1. Serine Hydrolase Selectivity : Kinetics and applications in organic and analytical chemistry

    Författare :Anders Hamberg; Karl Hult; Dick Janssen; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; monoacylation of diols; kinetic resolution; thermodynamics in enzyme catalysis; enzyme engineering; Biochemistry; Biokemi;

    Sammanfattning : The substrate selectivities for different serine hydrolases were utilized in various applications, presented in papers I-VI. The articles are discussed in the thesis in view of the kinetics of the enzyme catalysis involved. LÄS MER

  3. 2. Lipase chemoselectivity - kinetics and applications

    Författare :Cecilia Hedfors; Karl Hult; Mats Martinelle; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; Rhizomucor miehei lipase; active site titration; kinetics; chemoselectivity; thiol; alcohol; thiol-functionalized polyesters; Biochemistry; Biokemi;

    Sammanfattning :   A chemoselective catalyst is preferred in a chemical reaction where protecting groups otherwise are needed. The two lipases Candida antarctica lipase B and Rhizomucor miehei lipase showed large chemoselectivity ratios, defined as (kcat/KM)OH / (kcat/KM)SH, in a transacylation reaction with ethyl octanoate as acyl donor and hexanol or hexanethiol as acyl acceptor (paper I). LÄS MER

  4. 3. Lipase selectivity in functional polyester synthesis

    Författare :Cecilia Hedfors; Karl Hult; Romas Kazlauskas; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi;

    Sammanfattning : Enzyme selectivity means that the enzyme´s preferences towards competing substrates will be different. In this thesis, the enzyme selectivity has been studied for utilization in synthesis of functionalized macromonomers. LÄS MER

  5. 4. Lipase and ω-Transaminase : Biocatalytic Investigations

    Författare :Maria Svedendahl; Per Berglund; Stefan Stefan Lutz; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; enzyme catalysis; enzyme catalytic promiscuity; molecular modeling; ω-transaminase; Biochemistry; Biokemi;

    Sammanfattning : In a lipase investigation, Candida antarctica lipase B (CALB) are explored for enzyme catalytic promiscuity. Enzyme catalytic promiscuity is shown by enzymes catalyzing alternative catalytic transformations proceeding via different transition state structures than normal. LÄS MER

  7. 5. On electrostatic effects, minimal motion and other catalytic strategies used by enzymes

    Författare :Per-Olof Syrén; Karl Hult; Jiri Damborsky; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Enzymes are powerful biocatalysts that provide rate accelerations of up to 1019 fold compared to the corresponding uncatalyzed reaction in solution. The origin of the remarkable performance displayed by enzymes has fascinated and puzzled researchers for over a hundred years. LÄS MER