Sökning: "immunoaffinity"

Visar resultat 1 - 5 av 20 avhandlingar innehållade ordet immunoaffinity.

  1. 1. Biomarker detection via lab-on-a-chip integrated immunoaffinity approach for fluorescence and mass spectrometry readout

    Författare :Asilah Ahmad Tajudin; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immunoaffinity; affinity prefractionation; immunoMALDI; solid-phase extraction; protein digestion; ISET; antibody microarray; signal amplification; fluorescence; microfluidics; lab on a chip; separation; acoustophoresis; ultrasound; acoustic radiation force; plasma; proteomics; MALDI mass spectrometry; immunoassay; porous silicon.;

    Sammanfattning : The blood/plasma protein biomarker profile represents the pathological and physiological changes relating to human diseases. However, the plasma proteome reflects a high degree of complexity that results in a challenge for most analytical methods in clinical diagnostics. LÄS MER

  3. 2. Isolation of caveolae using affinity two-phase partitioning

    Författare :Parisa Abedinpour; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; basolateral; apical; immunoaffinity; wheat germ agglutinin; caveolin; caveolae caveolae enriched fraction; plasma membranes; rat lung; rat liver; Affinity Partitioning; two-phase partitioning;

    Sammanfattning : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. LÄS MER

  4. 3. Affinity Two-Phase Partitioning of Liposomes and Membranes

    Författare :Irene Barinaga-Rementeria Ramírez; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; miniaturised two-phase system; levitated drop; biotin; avidin; antibody; immunoaffinity; purification; rat lung; rat liver; caveolae; membrane; Affinity two-phase partitioning; liposome; Metabolism; Biochemistry; Biokemi; metabolism;

    Sammanfattning : Affinity two-phase partitioning based on an immunoaffinity sandwich approach for the rapid and selective purification of membranes is presented in this thesis. The method was developed by studying different parameters governing the affinity partitioning of model membranes. To this end, biotinylated liposomes were used. LÄS MER

  5. 4. Strategies for Miniaturized Biomarker Detection

    Författare :Belinda Adler; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

    Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER

  7. 5. Development of methods for determining aflatoxins in biological material

    Författare :Anders Kussak; Rainer Stephany; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Aflatoxins; Mycotoxins; Carcinogen; Airborne dust; Urine; Feed factories; Immunoaffinity extraction; Solid-phase extraction; Post-column derivatization; Electrospray; Mass spectrometry;

    Sammanfattning : In this thesis, it is shown how aflatoxins can be determined in biological material. The thesis is a summary of five papers.Aflatoxins are carcinogenic mycotoxins produced by Aspergillus moulds. Methods were developed for the determination of aflatoxins in samples of airborne dust and human urine collected at feed factories. LÄS MER