Sökning: "affinity"

Visar resultat 16 - 20 av 1392 avhandlingar innehållade ordet affinity.

  1. 16. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

    Författare :Gösta Lilius; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

    Sammanfattning : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. LÄS MER

  3. 17. Affibody ligands in immunotechnology applications

    Författare :Jenny Rönnmark; KTH; []
    Nyckelord :phage display; combinatorial; affinity; IgA ligand; immunohistochemistry; affibody-fusions;

    Sammanfattning : This thesis describes the development and use ofnon-immunoglobulin affinity proteins denoted affibodies asalternatives to antibodies in different immunotechnologyapplications. A 58 aa IgG Fc binding three-helix bundle domainZ, derived from staphylococcal protein A has been used asframework for library constructions, in which the face of themolecule involved in the native binding activity has beenengineered by combinatorial protein engineering. LÄS MER

  4. 18. Binding Kinetics of Proteins at Immune-Cell Contacts

    Författare :Manto Chouliara; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; T cells; Binding Kinetics; Affinity; Lifetime; CD4; CD2; TIRF Microscopy; SLBs;

    Sammanfattning : Protein-protein interactions are crucial in numerous cellular functions and biological processes that take place inside our body. It is therefore not surprising that these interactions also govern the response of our body´s defence mechanism, the so-called immune system, towards an infection. LÄS MER

  5. 19. Selective extraction systems for detection and purification of membrane proteins

    Författare :Ulf Sivars; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Sammanfattning : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. LÄS MER

  7. 20. Selective Sorbents - Silica-immobilized affinity ligands and molecularly imprinted polymers

    Författare :Magnus Glad; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular imprinting; HPLAC; composites; TRIM; Biokemi; Biochemistry; Metabolism; affinity chromatography;

    Sammanfattning : The development of selective sorbents for the separation of various molecules, preferentially biomolecules, is described in this work. Affinity ligands were immobilized on small silica particles and used in high-performance liquid affinity chromatography (HPLAC). LÄS MER