Sökning: "two-phase partitioning"

Visar resultat 1 - 5 av 33 avhandlingar innehållade orden two-phase partitioning.

  1. 1. Affinity Two-Phase Partitioning of Liposomes and Membranes

    Författare :Irene Barinaga-Rementeria Ramírez; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; miniaturised two-phase system; levitated drop; biotin; avidin; antibody; immunoaffinity; purification; rat lung; rat liver; caveolae; membrane; Affinity two-phase partitioning; liposome; Metabolism; Biochemistry; Biokemi; metabolism;

    Sammanfattning : Affinity two-phase partitioning based on an immunoaffinity sandwich approach for the rapid and selective purification of membranes is presented in this thesis. The method was developed by studying different parameters governing the affinity partitioning of model membranes. To this end, biotinylated liposomes were used. LÄS MER

  3. 2. Isolation of caveolae using affinity two-phase partitioning

    Författare :Parisa Abedinpour; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; basolateral; apical; immunoaffinity; wheat germ agglutinin; caveolin; caveolae caveolae enriched fraction; plasma membranes; rat lung; rat liver; Affinity Partitioning; two-phase partitioning;

    Sammanfattning : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. LÄS MER

  4. 3. Protein Surfaces Probed by Aqueous Two-phase Partitioning

    Författare :Kristina Berggren; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; partitioning; Aqueous two-phase systems; protein surface; peptide; Biochemistry; amino acid residue; Biokemi;

    Sammanfattning : This thesis is concerned primarily with peptide and protein partitioning in aqueous two-phase systems and the connection between partitioning behavior and protein structure. The surface properties of a protein is often crucial for recognition and interaction with other molecules. LÄS MER

  5. 4. Partitioning of biomolecules in thermoseparating polymer-water two-phase systems

    Författare :Hans-Olof Johansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aqueous two-phase system; temperature-induced phase separation; amino acid and peptide separation; polymer; partitioning; Biology; Biologi;

    Sammanfattning : The partitioning behaviour of different types of molecules in thermoseparating two-phase systems has been studied. A thermoseparating two phase system was formed by heating a water solution, which contained a thermoseparating polymer, above the cloud point temperature. LÄS MER

  7. 5. Peptide-tagged proteins in aqueous two-phase systems

    Författare :Anna Nilsson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; green fluorescent protein; cutinase; tryptophan; Aqueous two-phase system; peptide-tagged protein;

    Sammanfattning : This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. LÄS MER