Sökning: "Multiplexed assays"

Visar resultat 1 - 5 av 23 avhandlingar innehållade orden Multiplexed assays.

1. 1. On the application and validation of multiplexed affinity assays

   Författare :Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Bioteknologi; Biotechnology;

   Sammanfattning : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. LÄS MER

3. 2. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses

   Författare :Spyros Darmanis; Masood Kamali-Moghaddam; Ulf Landegren; Mathias Uhlen; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. LÄS MER

4. 3. Novel methods for improving rapid paper-based protein assays with gold nanoparticle detection

   Författare :Lara Lama; Helene Andersson Svahn; Jesper Gantelius; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Gold nanoparticles; ultrasound energy; signal enhancement; diagnostics; microarrays; paper-based assays; COMSOL Multiphysics simulations; Bioteknologi; Biotechnology;

   Sammanfattning : This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray assays. The assays utilize the optical characteristics of colloidal gold nanoparticles for the colorimetric detection of proteins.Multiplexed detection in sandwich immunoassays is limited by cross-reactivity between different detection antibodies. LÄS MER

5. 4. Proximity Ligation Assays for Disease Biomarkers Analysis

   Författare :Rachel Yuan Nong; Ulf Landegren; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; blood biomarkers; protein interactions; pathway analysis; single molecule; next-generation sequencing; Molekylär medicin; Molecular Medicine;

   Sammanfattning : One of the pressing needs in the field of disease biomarker discovery is new technologies that could allow high performance protein analysis in different types of clinical material, such as blood and solid tissues. This thesis includes four approaches that address important limitations of current technologies, thus enabling highly sensitive, specific and parallel protein measurements. LÄS MER

7. 5. Affinity assays for profiling disease-associated proteins in human plasma

   Författare :Sanna Byström; Jochen M. Schwenk; Claudia Fredolini; Peter Nilsson; Jennifer Van Eyk; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; affinity proteomics; immunoassay; antibody microarray; suspension bead array; protein profiling; immuno-capture; plasma; serum; cerebrospinal fluid; biomarker discovery; multiple sclerosis; malignant melanoma; mammographic breast density; Bioteknologi; Biotechnology;

   Sammanfattning : Affinity-based proteomics offers opportunities for the discovery and validation of disease-associated proteins in human body fluids. This thesis describes the use of antibody-based immunoassays for multiplexed analysis of proteins in human plasma, serum and cerebrospinal fluid (CSF). LÄS MER