Sökning: "metal quantification"

Visar resultat 1 - 5 av 29 avhandlingar innehållade orden metal quantification.

  1. 1. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Författare :Malin Mejàre; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER

  3. 2. Metal homeostasis as critical determinant for cellular fitness

    Författare :Jutta Diessl; Sabrina Büttner; Rajini Rao; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; metal homeostasis; manganese toxicity; coenzyme Q synthesis; mitochondrial respiration; calcineurin signaling; Pmr1; SPCA1; calcium; survival; caloric restriction; Parkinson’s disease models; proteotoxicity; Pep4; cathepsin D; molekylär biovetenskap; Molecular Bioscience;

    Sammanfattning : Metals play a crucial role in cellular biology. Bulk and trace metals such as calcium and manganese regulate a plethora of cellular processes ranging from signaling and oxidative stress to proteostasis and energy metabolism. LÄS MER

  4. 3. High-throughput protein analysis using mass spectrometry-based methods

    Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

  5. 4. The initial atmospheric corrosion of copper and zinc induced by carboxylic acids : Quantitative in situ analysis and computer simulations

    Författare :Harveth Gil; Christofer Leygraf; Johan Tidblad; Stuart Lyon; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Atmospheric corrosion; copper; zinc; carboxylic acids; modeling; GILDES; in situ; quantification;

    Sammanfattning : Degradation of metals through atmospheric corrosion is a most important and costly phenomenon with significant effects on, e.g., the lifespan of industrial materials, the reliability of electronic components and military equipment, and the aesthetic appearance of our cultural heritage. LÄS MER

  7. 5. Effect of shear cutting on metal fatigue

    Författare :David Gustafsson; Erik Olsson; Jörgen Kajberg; Pär Jonsén; Henrik Sieurin; Luleå tekniska universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Punching; Trimming; Shear cutting; Fatigue; HCF; Residual stresses; Hållfasthetslära; Solid Mechanics;

    Sammanfattning : Lightweighting of automotive and heavy-duty vehicle components is an important task that does not need any further motivation or background. It can be read in a large part of the technical papers in the field. A common approach for finding lighter solutions is to increase the material grade while decreasing the material thickness. LÄS MER