Sökning: "weak affinity chromatography"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden weak affinity chromatography.

  1. 1. Insights into weak affinity antibody-antigen interactionsStudies using affinity chromatography and optical biosensor

    Författare :Magnus Strandh; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; Biotechnology; immunosensor; biosensor; weak affinity chromatography; cross-reactivity; antibody-antigen interaction; weak affinity; biomolecular recognition; Immunology; serology; transplantation; Immunologi; serologi;

    Sammanfattning : Molecular recognition and interaction is fundamental for the function of biological systems. The properties of the interacting biomolecules dictate the type of forces involved and the strength and dynamics of the interaction. Many interactions are very strong whereas others exhibit weak affinity. LÄS MER

  3. 2. Introducing weak affinity chromatography to drug discovery with focus on fragment screening

    Författare :Minh-Dao Duong-Thi; Sten Ohlson; Brian Dymock; Linnéuniversitetet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity LC-MS; fragment-based drug discovery; fragment screening; high throughput; mass spectrometry; stereoisomer; enantiomer; thrombin; weak affinity chromatography; WAC; WAC-MS.; Biomedical Sciences; Biomedicinsk vetenskap; Biokemi; Biochemistry;

    Sammanfattning : Fragment-based drug discovery is an emerging process that has gained popularity in recent years. The process starts from small molecules called fragments. One major step in fragment-based drug discovery is fragment screening, which is a strategy to screen libraries of small molecules to find hits. LÄS MER

  4. 3. Progress of Weak Affinity Chromatography as a Tool in Drug Development

    Författare :Elinor Meiby; Sten Ohlson; Manfred Raida; Linnéuniversitetet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; weak affinity chromatography; fragment based drug discovery; fragment screening; thrombin; trypsin; cyclin G-associated kinase; Hsp90; Pin1; cyclooxygenase; lipodisks; Biokemi; Biochemistry;

    Sammanfattning : Weak Affinity Chromatography (WAC) is a technology that was developed to analyse weak (KD > 10-5 M) although selective interactions between biomolecules. The focus of this thesis was to develop this method for various applications in the drug development process.Fragment Based Drug Discovery is a new approach in finding new small molecular drugs. LÄS MER

  5. 4. Development of low affinity techniques for applications in clinical chemistry

    Författare :Lisa Leickt; Avdelningen för klinisk kemi och farmakologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; chromatography performance; acute myocardial infarction; antibodies; creatine kinase MB isoforms; lectins; low affinity interactions; weak affinity chromatography; Clinical chemistry; Klinisk kemi;

    Sammanfattning : An increase in the plasma level of creatine kinase MB (CKMB) has long been used to indicate acute myocardial infarction (AMI). For optimal treatment an early diagnosis is important. An increase in the ratio between the two isoforms of CKMB is seen much earlier than an increase in the total level of CKMB. LÄS MER

  7. 5. Development of Flourescence-based Immunosensors for Continous Carbohydrate Monotoring : Applications for Maltose and Glucose

    Författare :Henrik Engström; Sten Ohlson; Per Ola Andersson; Christopher Lowe; Högskolan i Kalmar; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Carbohydrate; Diabetes; Immunosensor; ; Glucose; Maltose; Weak affinity chromatograhpy; Kolhydrater; Glukos; Maltose; Affinitetskromatografi; Diabetes; Biochemistry; Biokemi; Bioteknik; Biotechnology;

    Sammanfattning : Weak affinity interaction of monoclonal antibodies and carbohydrate antigens can be detected and quantified by alterations in the antibodies' intrinsic tryptophan fluorescence. These weak/transient binding events have been monitored by total internal reflection fluorescence (TlRF) by facilitating the change in intrinsic tryptophan fluorescence. LÄS MER