Sökning: "protein purification"

Visar resultat 1 - 5 av 245 avhandlingar innehållade orden protein purification.

  1. 1. Recombinant amelogenin - Strategies for purification and protein processing

    Författare :Johan Svensson Bonde; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

    Sammanfattning : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. LÄS MER

  2. 2. Engineering of small IgG binding domains for antibody labelling and purification

    Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Biotechnology; Bioteknologi;

    Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER

  3. 3. Adipocyte phosphatases and the antilipolytic action of insulin

    Författare :Svante Resjö; Signaltransduktionsforskning; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein phosphorylation; protein purification; adipose tissue; PP2A; protein phosphatase; PDE3B; phosphodiesterase 3B; Insulin signalling; antilipolysis; Klinisk biologi; Clinical biology; PKB; protein kinase B;

    Sammanfattning : Adipose tissue is the main site of energy storage of the body and an important endocrine organ. Knowledge of the regulation of fat metabolism and the endocrine factors secreted by the adipocyte is crucial for the understanding of diseases such as obesity and diabetes. Insulin is the main antilipolytic hormone. LÄS MER

  4. 4. Human Aquaporins: Production, Characterization and Interactions

    Författare :Jennie Sjöhamn; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Nyckelord :aquaporins; membrane protein; protein production; protein characterization; protein:protein interactions; structural biochemistry;

    Sammanfattning : Membrane proteins are essential components of the cell and responsible for the communication with the outside environment and transport of molecules across the membrane. Water transport is facilitated by aquaporins, which are water selective transmembrane pores that serve to maintain cell homeostasis. LÄS MER

  5. 5. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Författare :Malin Mejàre; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER