Sökning: "labeling enzymes"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden labeling enzymes.

1. 1. New Bioanalytical Approaches, Magneto Immunoassays and SIRE Technology Based Biosensors

   Författare :Kirstin Kriz; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemical technology; Biokemisk teknik; antigens; superparamagnetic nanoparticles; magnetic inductance transducer; competitive magneto immunoassay; enzymes; metabolites; differential measurements; SIRE; biosensor;

   Sammanfattning : This thesis presents two new technologies, SIRE technology based biosensors and magneto immunoassays, which can be used for bioanalytical applications. One of the main problems associated with biosensor technology is the instability of the recognition element since it is of biological origin and thus, has a limited lifetime. LÄS MER

3. 2. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

   Författare :Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
   Nyckelord :antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. LÄS MER

4. 3. Labeling, tagging and charging - Long standing issues in bioenergetics addressed with novel techniques

   Författare :Tobias Gustavsson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; WrbA; site specific labeling; fusion proteins; spectro electrochemistry; succinate quinone reductase; NADH quinone oxidoredictase; cytochromes; Respiratory chain;

   Sammanfattning : Understanding the molecular details of bioenergetic processes is fundamentally important. Defects in the mitochondrial energy metabolism can lead to untimely apoptosis and thus cause serious degenerative human diseases. LÄS MER

5. 4. Modulating Sirtuin Activity - Design, Synthesis and Evaluation of Sirtuin 2 Inhibitors

   Författare :Tina Seifert; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Sirtuin; SIRT2; Inhibitors; Chroman-4-ones; Chromones; Benzothiadiazine-1; 1-dioxides; Saccharin; Scaffold; Structure-activity relationship; Antiproliferative properties; Binding site; Homology modeling; Photoaffinity labeling; Diazirine; Mass spectrometry;

   Sammanfattning : Sirtuins (SIRTs) are NAD+-dependent lysine deacetylating enzymes targeting histones and a multitude of non-histone proteins. The SIRTs have been related to important cellular processes such as gene expression, cell proliferation, apoptosis and metabolism. They are proposed to be involved in the pathogenesis of e.g. LÄS MER

7. 5. Fluorescent Probes and Protein Misfolding: Methods and Applications

   Författare :Irem Nasir; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein misfolding; fluorophores; filter-trap; site-specific fluorophore labeling; amyloid-beta; microscopic rate constants; solvatochromic probes; alpha-synuclein; lipid vesicles; nanoparticles; protein adsorption;

   Sammanfattning : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. LÄS MER