Sökning: "site-specific fluorophore labeling"

Hittade 2 avhandlingar innehållade orden site-specific fluorophore labeling.

1. 1. Fluorescent Probes and Protein Misfolding: Methods and Applications

   Författare :Irem Nasir; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein misfolding; fluorophores; filter-trap; site-specific fluorophore labeling; amyloid-beta; microscopic rate constants; solvatochromic probes; alpha-synuclein; lipid vesicles; nanoparticles; protein adsorption;

   Sammanfattning : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. LÄS MER

3. 2. A Novel Route for Construction of Multipurpose Receptors through Chemical Modification of Glutathione Transferases

   Författare :Johan Viljanen; Bengt Harald Jonsson; Kerstin Broo; Sophia Hober; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Human GST A1-1; site-specific covalent modification; tyrosine 9; lysine 216; methionine 208; multipurpose receptor; pattern recognition; protein purification; Chemistry; Kemi;

   Sammanfattning : This thesis describes how the human Alpha class glutathione transferase (GST) A1-1 can be reprogrammed either to function as a multipurpose biosensor for detection of small molecule analytes, or as a handle providing for more efficient protein purification.A novel, user-friendly, and efficient method for site-specific introduction of functional groups into the active site of hGST A1-1 is the platform for these achievements. LÄS MER