Sökning: "Z domain"

Visar resultat 1 - 5 av 53 avhandlingar innehållade orden Z domain.

1. 1. Dynamic Source Models of Icelandic Earthquakes and Teleseismic Tomograhy along the TOR array

   Författare :Z. Hossein Shomali; Göran Ekström; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Earth sciences; Geovetenskap; Earth sciences; Geovetenskap; Geofysik med inriktning mot seismologi; Geophysics with specialization in Seismology;

   Sammanfattning : This thesis describes new inversion-oriented methodological developments and their seismological applications. In the first study presented the dynamic source parameters of some local Icelandic earthquakes are studied by employing a time domain moment tensor inversion method. LÄS MER

3. 2. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

   Författare :Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
   Nyckelord :antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. LÄS MER

4. 3. Protein engineering to explore and improve affinity ligands

   Författare :Martin Linhult; KTH; []
   Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

   Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

5. 4. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

   Författare :Cynthia Hassinen; KTH; []
   Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

   Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

7. 5. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

   Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

   Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER