Sökning: "SRA - Molekylär biovetenskap"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden SRA - Molekylär biovetenskap.

  1. 1. Ribosome display for selection and evolution of affibody molecules

    Författare :Sebastian Grimm; Per-Åke Nygren; Mike Taussig; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; ribosome display; phage display; combinatorial protein engineering; library; murine IgG1; SATB1; Ras; Raf; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : Affinity proteins are invaluable tools in biotechnological and medical applications. This thesis is about combinatorial protein engineering principles for the generation of novel affinity proteins to purify mouse immunoglobulin, detect a potential cancer marker protein or inhibit a cell proliferation pathway. LÄS MER

  2. 2. Engineering of Affibody molecules for Radionuclide Molecular Imaging and Intracellular Targeting

    Författare :Camilla Hofström; Torbjörn Gräslund; Serge Muyldermans; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; Affibody molecules; affinity proteins; radionuclide molecular imaging; intracellular targeting; EGFR; HER2; IGF-1R; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : Affibody molecules are small (7 kDa) affinity proteins of non-immunoglobulin origin that have been generated to specifically interact with a large number of clinically important molecular targets.In this thesis, Affibody molecules have been employed as tracers for radionuclide molecular imaging of HER2- and IGF-1R-expressing tumors, paper I-IV, and for surface knock-down of EGFR, paper V. LÄS MER

  3. 3. Intracellular systems for characterization and engineering of proteases and their substrates

    Författare :George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. LÄS MER

  4. 4. On the Design of Affibody Molecules for Radiolabeling and In Vivo Molecular Imaging

    Författare :Daniel Rosik; Amelie Eriksson Karlström; Christian Heinis; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; AC DC; radionuclide molecular imaging; HER2; SPECT; PET; biodistribution; peptide synthesis; radiolabeling; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : Affibody molecules have lately shown great potential as tools for in vivo molecular imaging. These small, 3-helical bundles, with their highly stable protein scaffold, are well suited for the often harsh conditions of radiolabeling. LÄS MER

  5. 5. Theoretical studies on photophysics and photochemistry of DNA

    Författare :Yuejie Ai; Yi Luo; Lluís Blancafort; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Quantum chemistry; Kvantkemi; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Sammanfattning : Theoretical studies on biological systems like nucleic acid and protein have been widely developed in the past 50 years and will continue to be a topic of interest in forefronts of natural science. In addition to experimental science, computational modeling can give useful information and help us to understand biochemical issues at molecular, atomic and even electronic levels. LÄS MER