Sökning: "calcium binding"

Visar resultat 1 - 5 av 204 avhandlingar innehållade orden calcium binding.

1. 1. Studies of calcium binding proteins 1 and 2 and protein kinase CK2

   Författare :Inger Janson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; calcium binding protein 1; calcium binding protein 2; calcium binding protein 4; erd2receptor; protein kinase CK2; endoplasmic reticulum; Golgi; microsomes; heparin; Biokemi; Biochemistry; Biokemi; Medical and Physiological Chemistry; medicinsk och fysiologisk kemi;

   Sammanfattning : The endoplasmic reticulum (ER) is a major intracellular compartment of eukaryotic cells and is the site of the synthesis and post-translational modification of both secretory and membrane glycoproteins. Upon cell rupture vesicles of several kinds are formed from cell membranes, mainly derived from ER and are called microsomes. LÄS MER

3. 2. Calreticulins- Calcium-Binding Proteins with Functional Diversity and Evolutionary Duality

   Författare :Staffan Persson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Plant biochemistry; plant; isoforms; evolution; diversity; differential regulation; chaperone; calnexin; calmegin; calcium; Arabidopsis; calreticulin; Växtbiokemi;

   Sammanfattning : Calreticulin is a multifunctional protein mainly localized in the endoplasmic reticulum in eukaryotic organisms, except yeasts. The protein comprises three distinct regions: the N-terminal N domain, the C-terminal C domain, and the central P domain, reflecting the functional diversity of calreticulin. LÄS MER

4. 3. Shaking the EF-Hand. Dynamics and Ion-Binding to Calmodulin and Calbindin D9k

   Författare :Anders Malmendal; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; magnesium; line shape; ion binding; EF-hand; cooperativity; conformational exchange; chemical shift; calmodulin; calcium; calbindin D9k; allostery; backbone dynamics; NMR; relaxation; Physical chemistry; Fysikalisk kemi;

   Sammanfattning : The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. The x-ray structure of magnesium-saturated calbindin D9k shows a magnesium ion in only one of the two ion binding sites. LÄS MER

5. 4. Protein Interactions: Electrostatics and Ligand Binding

   Författare :Ingemar André; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biophysics; Molekylär biofysik; secondary structure formation; protein GB1; pKa values; pH stability; NMR; electrostatic interactions; M proteins; calbindin D9k; calcium binding; cooperativity; calmodulin; calbindin D28k;

   Sammanfattning : This thesis deals with Ca2+ binding to proteins, electrostatic interactions in and between proteins as well as inter- and intramolecular interactions. A computer program was developed to determine Ca2+ binding constants from experimental titration data of proteins. LÄS MER

7. 5. Calcium transporting ATPases in plant cells

   Författare :Malin Olbe; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Triticum aestivum; calcium; Brij 58; calmodulin; Ca2 signalling; inside-out; endoplasmic reticulum; plant; plasma membrane; proteolysis; P-type ATPase; spinach; Spinacea oleracea; trypsin; two-phase; wheat.; Plant biochemistry; Växtbiokemi;

   Sammanfattning : Plant cells carry Ca2+ ATPases to maintain the low resting level of cytosolic Ca2+ (0.1-0.2 µM) found in all eukaryotes. Plant Ca2+ ATPases are mainly located in the plasma membrane, the endoplasmic reticulum and in the vacuolar membrane, and consequently large Ca2+ gradients are created across these membranes. LÄS MER