Sökning: "EF-hand"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet EF-hand.

  1. 1. Exploring the interactome of EF-hand proteins

    Författare :Mikael Bauer; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; EF-hand; calcium; STIM; stromal interaction molecule; secretagogin; Calmodulin; calbindin D28k; protein-protein interactions; zinc; protein array;

    Sammanfattning : EF-hand proteins are central in many cellular processes being the main group of Ca2+-sensing and buffering proteins in the cell. In the work presented in this thesis we study ligand interactions of three EF-hand proteins; calmodulin, calbindin D28k and secretagogin. LÄS MER

  3. 2. Shaking the EF-Hand. Dynamics and Ion-Binding to Calmodulin and Calbindin D9k

    Författare :Anders Malmendal; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; magnesium; line shape; ion binding; EF-hand; cooperativity; conformational exchange; chemical shift; calmodulin; calcium; calbindin D9k; allostery; backbone dynamics; NMR; relaxation; Physical chemistry; Fysikalisk kemi;

    Sammanfattning : The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. The x-ray structure of magnesium-saturated calbindin D9k shows a magnesium ion in only one of the two ion binding sites. LÄS MER

  4. 3. The effect of sequence truncation and amino-acid substitutions on the biophysical properties of protein S and calbindin D9k

    Författare :Karin Julenius; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; surface plasmon resonance; fluorescence spectroscopy; circular dichroism spectroscopy; NMR spectroscopy; hydrophobic effect; protein stability; dimerization; cooperativity; calcium binding; EGF-module; EF-hand; calbindin D9k; protein S; synthet; Physical chemistry; Fysikalisk kemi;

    Sammanfattning : Protein S is a modular protein and a cofactor in the protein C anticoagulant system. It consists of a Gla-module, a thrombin-sensitive loop, four EGF-modules and a SHBG-like module. LÄS MER

  5. 4. Protein Dynamics Studied by NMR Spin Relaxation. Conformational Transitions of a Calmodulin Mutant

    Författare :Patrik Lundström; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molekylär biofysik; Molecular biophysics; spin relaxation; rotating-frame; NMR; multiple-quantum; EF-hand; chemical shift; chemical exchange; calcium binding proteins; calmodulin;

    Sammanfattning : Binding of calcium to the protein calmodulin leads to molecular reorganization that enables interaction with target peptides and activation of downstream processes. I have studied the dynamics of the calcium-loaded form of a C-terminal calmodulin mutant (E140Q-Tr2C) using NMR spin relaxation experiments. LÄS MER

  7. 5. Structural studies of metal-binding proteins revealing dynamic behaviour

    Författare :Maria Håkansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kemi; Chemistry; Molekylär biofysik; Molecular biophysics; zinc; magnesium; superantigen; calbindin D9k; X-ray crystallography; staphylococcal enterotoxin; structure; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

    Sammanfattning : The Mg2+ and Mn2+ structures of calbindin D9k have been determined and they reveal a conformational change compared to the structures of apo and (Ca2+)2-calbindin D9k. These findings are unique, since normally calcium and not magnesium is known to induce conformational changes of EF-hand proteins. LÄS MER