Sökning: "Medical Biochemistry"

Visar resultat 36 - 40 av 455 avhandlingar innehållade orden Medical Biochemistry.

  1. 36. Functional characterization of the human adenovirus pVII protein and non-coding VA RNAI

    Författare :Raviteja Inturi; Tanel Punga; Goran Akusjarvi; Thomas Dobner; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Adenovirus; VA RNA; protein VII; Ubiquitination; proteasome; anti-viral; Medical Biochemistry; Medicinsk biokemi; Mikrobiologi; Microbiology; Medical Virology; Medicinsk virologi;

    Sammanfattning : Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. LÄS MER

  3. 37. Activity of tumor-antigens in polyomavirus replication

    Författare :Hongyun Li; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Tumor-Antigens; DNA Replication; Cell-cycle; Phosphorylation; J-domain; Biokemi; Biochemistry; Biokemi; Medical Virology; medicinsk virologi;

    Sammanfattning : Polyomavirus replication needs an active cell environment, and the viral DNA replication is cell cycle regulated. Tumor antigens (T-antigens) of mouse polyomavirus, especially large T-antigen, are necessary for the replication. LÄS MER

  4. 38. Adenovirus vector systems permitting regulated protein expression and their use for in vivo splicing studies

    Författare :Magnus Molin; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Adenovirus; vector; inducible; gene expression; ASF SF2; splicing; Biokemi; Biochemistry; Biokemi; Medical Virology; medicinsk virologi;

    Sammanfattning : We have constructed two adenovirus-based gene expression vector systems permitting regulated protein expression. They are based on the tetracycline-regulated Tet-ON- and the progesterone antagonist RU 486-regulated gene expression systems, which were rescued into E1-deficient adenovirus vectors. LÄS MER

  5. 39. DNA and protein interactions of mouse polyomavirus large T antigen

    Författare :Ola Rönn; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; T-antigen; DNA-binding; biosensor; enhancer; Biokemi; Biochemistry; Biokemi; Medical Virology; medicinsk virologi;

    Sammanfattning : Polyomaviruses require dividing cells to multiply. The regulatory genes express proteins, theT-antigens, necessary for this to occur. Large T-antigen (LT), is a phosphoprotein withmultiple intrinsic activities, e.g. LÄS MER

  7. 40. High-throughput screening using multicellular tumor spheroids to reveal and exploit tumor-specific vulnerabilities

    Författare :Wojciech Senkowski; Mårten Fryknäs; Rolf Larsson; Peter Nygren; Mats Gustafsson; Krister Wennerberg; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; spheroids; high-throughput screening; nitazoxanide; OXPHOS; gene expression profiling; statins; drug repositioning; 3D cell culture; quiescent cells; Medical Science; Medicinsk vetenskap;

    Sammanfattning : High-throughput drug screening (HTS) in live cells is often a vital part of the preclinical anticancer drug discovery process. So far, two-dimensional (2D) monolayer cell cultures have been the most prevalent model in HTS endeavors. However, 2D cell cultures often fail to recapitulate the complex microenvironments of in vivo tumors. LÄS MER