Sökning: "soluble protein"

Visar resultat 21 - 25 av 444 avhandlingar innehållade orden soluble protein.

  1. 21. Studies on spinach plastocyanin and mutants : Expression in Escherichia coli, folding and function

    Författare :Mikael Ejdebäck; Örjan Hansson; Miguel A. De la Rosa; Göteborgs Universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; plastocyanin; electron transfer; copper; protein-protein interactions; protein expression; folding; site-directed mutagenesis; NMR; Naturvetenskap; Natural sciences;

    Sammanfattning : Photosynthesis is a process in which photons from sunlight excite chlorophylls in the thylakoid membranes of plants, algae and cyanobacteria. The photo-oxidised reaction centre chlorophyll P700 is re-reduced by an electron transferred from the soluble, blue copper protein plastocyanin. LÄS MER

  3. 22. Misfolded superoxide dismutase-1 in amyotrophic lateral sclerosis

    Författare :Per Zetterström; Stefan L Marklund; Lawrence J. Hayward; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ALS; SOD1; protein misfolding; SOD1 conformation; disulfide-reduced; transgenic mice; cerebrospinal fluid; protein-protein interaction; antibodies; Clinical chemistry; Klinisk kemi; Clinical Chemistry; klinisk kemi;

    Sammanfattning : Amyotrophic lateral sclerosis (ALS) is a disease in which the motor neurons die in a progressive manner, leading to paralysis and muscle wasting. ALS is always fatal, usually through respiratory failure when the disease reaches muscles needed for breathing. Most cases are sporadic, but approximately 5–10% are familial. LÄS MER

  4. 23. Staphylococcal surface display for protein engineering and characterization

    Författare :John Löfblom; Stefan Ståhl; Henrik Wernérus; K. Dane Wittrup; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; affibody; combinatorial library; epitope mapping; Gram-positive bacteria; protein engineering; staphylococcal surface display; Molecular biology; Molekylärbiologi;

    Sammanfattning : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. LÄS MER

  5. 24. Diffusive interactions play an important role in protein stability and mobility : Investigations of the intracellular milieu using in-cell NMR

    Författare :Therese Sörensen; Mikael Oliveberg; Per Jemth; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; in-cell NMR; protein-protein interactions; mobility; stability; thermodynamics; Nuclear Magnetic Resonance; stopped-flow spectroscopy; proteins; SOD1; HAH1; TTHA1718; cells; cytoplasm; humans; bacteria; ions; polyions; electrostatics; biokemi; Biochemistry;

    Sammanfattning : Proteins are crucial for all cellular life. Every signal received by a cell, and every response to it, is mediated by proteins. Inside cells, these proteins diffusively sample each other’s surfaces, as they travel through the cytoplasm in search of their specific interaction partners. LÄS MER

  7. 25. Enhancing the therapeutic effect of biological drugs with protein engineering : Focusing on pre-clinical Alzheimer’s disease therapy

    Författare :Fadi Rofo; Greta Hultqvist; Stina Syvänen; Sara Mangsbo; Joy Zuchero; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alzheimer’s disease; amyloid-beta; blood-brain barrier; transferrin receptor; protein-drugs; multivalent-bispecific antibodies;

    Sammanfattning : Aggregation of the amyloid-β peptide (Aβ) is one of the main pathological hallmarks in Alzheimer’s disease (AD). The soluble Aβ aggregates (oligomers and protofibrils) have shown to be the most harmful species. Hence, targeting these aggregates can be of therapeutic potential. LÄS MER