Sökning: "John Löfblom"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden John Löfblom.

1. 1. Staphylococcal surface display for protein engineering and characterization

   Författare :John Löfblom; Stefan Ståhl; Henrik Wernérus; K. Dane Wittrup; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; affibody; combinatorial library; epitope mapping; Gram-positive bacteria; protein engineering; staphylococcal surface display; Molecular biology; Molekylärbiologi;

   Sammanfattning : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. LÄS MER

3. 2. Combinatorial Protein Engineering Of Affibody Molecules Using E. Coli Display And Rational Design Of Affibody-Based Tracers For Medical Imaging

   Författare :Ken G. Andersson; John Löfblom; Stefan Ståhl; Harald Kolmar; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; directed evolution; microbial display; E. coli; Affibody molecule; autotransporter; medical imaging; HER receptor family; Sortase A; Bioteknologi; Biotechnology;

   Sammanfattning : Directed evolution is today an established strategy for generation of new affinity proteins. This thesis describes the development of a cell-display method using Escherichia coli for directed evolution of Affibody molecules. LÄS MER

4. 3. Affibody-mediated targeting of HER-family receptors for cancer imaging and therapy

   Författare :Charles Dahlsson Leitao; Stefan Ståhl; John Löfblom; Christoph Rader; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cancer therapy; radionuclide imaging; affibody molecules; HER-receptors; prodrug; protein design; Cancerterapi; radionuklidavbildning; affibody molekyler; HER-receptorer; prodrog; proteindesign; Bioteknologi; Biotechnology;

   Sammanfattning : Proteins are remarkable molecules with diverse and specialized functions playing essential roles in most biological processes. One such function is protecting us from diseases by the action of antibodies in our immune system that can recognize and mediate the destruction of invading pathogens by binding to foreign epitopes found on non-self proteins. LÄS MER

5. 4. Bacterial display systems for engineering of affinity proteins

   Författare :Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
   Nyckelord :Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

   Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER

7. 5. Affibody Molecules Targeting VEGFR2 - Two Turns Off and Four Turns On

   Författare :Rezan Güler; John Löfblom; Stefan Ståhl; Dario Neri; KTH; []
   Nyckelord :Protein engineering; affibody molecules; VEGFR2; angiogenesis; Bioteknologi; Biotechnology;

   Sammanfattning : The notion of employing proteins as drugs traces back several decades. As recombinant DNA technology emerged, it became a powerful tool for the tailoring of protein traits via genetic approaches - so-called protein engineering. LÄS MER