Sökning: "in-cell NMR"

Hittade 5 avhandlingar innehållade orden in-cell NMR.

  1. 1. Protein stability and mobility in live cells : Revelation of the intracellular diffusive interaction organization mechanisms

    Författare :Xin Mu; Mikael Oliveberg; Johan Elf; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; diffusive interactions; macromolecular crowding; protein thermodynamic stability; protein mobility; in-cell NMR; Hofmeister series; biokemi; Biochemistry;

    Sammanfattning : Biochemical processes inside living cells take place in a confined and highly crowded environment. As such, macromolecular crowding, one of the most important physicochemical properties of cytoplasm, is an essential element of cell physiology. LÄS MER

  2. 2. Effect of Ischaemia-Reperfusion on Rabbit Kidney and Human Brain

    Författare :Jagdish Shivlal Gondalia; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Nyckelord :Free radicals; ischaemia-reperfusion; cell bioenergetics; NMR; ESR; carotid artery stenosis; endarterectomy; allopurinol; acetylcysteine; MMP-1; ICAM-1; MCP-9; oxLDL;

    Sammanfattning : Free radicals are produced in various organs at ischaemia-reperfusion. The final stage in radical damage is lipid peroxidation. We have demonstrated previously that a lipid-soluble antioxidant improves restoration of bioenergetics in rabbit kidneys after ischaemia, as reflected in 31P spectrometry. LÄS MER

  3. 3. Biochemical and Structural Studies on the Adaptor Protein p130Cas

    Författare :Fariborz Nasertorabi; Kathryn R Ely; Lars Liljas; Jyrki Heino; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Integrin signaling; p130Cas; X-ray Crystallography; NMR Spectroscopy; Protein phosphorylation; BCAR-1; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi;

    Sammanfattning : Crk associated substrate (Cas) is an adaptor protein that becomes phosphorylated upon integrin signaling and influences regulation of cell processes such as migration, proliferation and survival. It consists of multiple domains and regions that can interact with several signaling proteins involved in different signaling pathways. LÄS MER

  4. 4. SOD1 Aggregation : Relevance of thermodynamic stability

    Författare :Lisa Lang; Mikael Oliveberg; Michele Vendruscolo; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Aggregation; misfolding; Superoxide dismutase SOD1 ; Amyotrophic lateral sclerosis ALS ; unfolding; thermodynamic stability; biokemi; Biochemistry;

    Sammanfattning : Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease affecting the upper and lower motor neurons causing muscle atrophy and paralysis followed by death. Aggregates containing superoxide dismutase (SOD1) are found as pathological hallmark in diseased ALS patients. LÄS MER

  5. 5. Isolation and characterization of regulatory peptides and bioactive compounds

    Författare :Åke Norberg; Karolinska Institutet; Karolinska Institutet; []

    Sammanfattning : Isolation of peptides and other bioactive compounds is an important and often necessary step to get the total information about their structures. This is demonstrated by a number of different characterizations in this thesis. LÄS MER