Sökning: "lysate screening"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden lysate screening.

  1. 1. High-throughput protein analysis using mass spectrometry-based methods

    Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

  3. 2. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity

    Författare :Olof Modén; Bengt Mannervik; Mikael Widersten; Kenneth Tew; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; allelic variants; azathioprine; bioactivation; chimeric mutagenesis; directed evolution; DNA shuffling; enzyme engineering; glutathione transferase; GST; lysate screening; molecular docking; multiple alignment; multivariate analysis; polymorphism; principal component analysis; prodrug; prodrug activation; protein engineering; protein redesign; reduced amino acid alphabet; saturation mutagenesis; semi-rational enzyme engineering; site-directed mutagenesis; structure-activity relationship; structure-based redesign; Biokemi; Biochemistry;

    Sammanfattning : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. LÄS MER

  4. 3. Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

    Författare :Amro Hanora; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Downstream processing; macroporous; Biologically active compounds; Biotechnology; Läkemedelsteknik och relaterad teknik; Pharmaceutical and related technologies; Polymerteknik; biopolymers; Biokemisk teknik; Polymer technology; Makromolekylär kemi; Biochemical technology; Affinity chromatography; Ion exchange chromatography; Expanded bed adsorption chromatography.; Macromolecular chemistry; High through put screening; lactica acids; Endotoxin; Plasmid DNA; Cryogel columns; Bioteknik;

    Sammanfattning : The completion of sequencing of human and other mammalian genomes opens a new era for drug development. New approaches have been proposed for tackling diseases such as development of small molecule therapies based on understanding of human genome and proteome, new proteinous based therapies, where the therapeutic protein can inhibit or modify a certain metabolic pathway or DNA-based therapies delivering a gene into the cell (e. LÄS MER

  5. 4. Structural Studies on PP2A and Methods in Protein Production

    Författare :Auður Magnúsdóttir; Pär Nordlund; Herman Van Tilbeurgh; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PP2A; PTPA; B56γ; Protein Production; Structural biology; Strukturbiologi; biokemi; Biochemistry;

    Sammanfattning : PP2A is a major phosphatase in the cell that participates in multiple cell signaling pathways. It is a heterotrimer of a core dimer and variable regulatory subunits. Details of its structure, function and regulation are slowly emerging. Here, the structure of two regulators of PP2A are de-scribed; PTPA and B56γ. LÄS MER

  7. 5. Shining light on unknown function proteins

    Författare :Riccardo Diamanti; Martin Högbom; Hans-Petter Hersleth; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteins; membrane proteins; metalloproteins; biochemistry; metabolism; biokemi; Biochemistry;

    Sammanfattning : The study of protein structure and function is a fundamental step for understanding biological systems and for creating a solid ground for future drug development. Despite the development of novel ground-breaking biochemical and bioinformatical tools in the last decades, most proteins found in nature still have unknown assigned function. LÄS MER