Sökning: "Expanded bed adsorption chromatography."

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden Expanded bed adsorption chromatography..

1. 1. Fast isolation of proteins from bioreactors using affinity chromatography techniques

   Författare :Eva Pålsson; Avdelningen för klinisk genetik; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chromatography; protein purification; analyses; dispersion; chromatography matrices; Biochemistry; Biokemi; Metabolism; expanded bed; metabolism; Biotechnology; Bioteknik;

   Sammanfattning : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. LÄS MER

3. 2. Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

   Författare :Amro Hanora; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Downstream processing; macroporous; Biologically active compounds; Biotechnology; Läkemedelsteknik och relaterad teknik; Pharmaceutical and related technologies; Polymerteknik; biopolymers; Biokemisk teknik; Polymer technology; Makromolekylär kemi; Biochemical technology; Affinity chromatography; Ion exchange chromatography; Expanded bed adsorption chromatography.; Macromolecular chemistry; High through put screening; lactica acids; Endotoxin; Plasmid DNA; Cryogel columns; Bioteknik;

   Sammanfattning : The completion of sequencing of human and other mammalian genomes opens a new era for drug development. New approaches have been proposed for tackling diseases such as development of small molecule therapies based on understanding of human genome and proteome, new proteinous based therapies, where the therapeutic protein can inhibit or modify a certain metabolic pathway or DNA-based therapies delivering a gene into the cell (e. LÄS MER

4. 3. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

   Författare :Cynthia Hassinen; KTH; []
   Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

   Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

5. 4. Polyelectrolytes and Polycomplexes in Biotechnology

   Författare :Maria Dainiak; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; expanded bed adsorption.; affinity precipitation; Fab fragments; monoclonal antibodies; glyceraldehyde-3-phosphate dehydrogenase; misfolded protein; chaperone; poly N-ethyl-4-vinylpyridinium bromide ; poly methacrylic acid ; poly acrylic acid ; Key words: polyelectrolyte; polyelectrolyte complexes; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

   Sammanfattning : Polyelectrolytes and polyelectrolyte complexes (PECs) are characterized by many useful properties that endow them with high potential in different areas of biotechnology. This thesis describes the development and analyses of some new systems and methods utilizing poly(acrylic acid) (PAA), poly(methacrylic acid) (PMA), poly (N-ethyl-4-vinylpyridinium bromide) (PEVP) and PMA-PEVP complexes. LÄS MER

7. 5. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

   Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

   Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER