Sökning: "Protein Production"

Visar resultat 1 - 5 av 1301 avhandlingar innehållade orden Protein Production.

  1. 1. Proteome wide protein production

    Författare :Hanna Tegel; Sophia Hober; Diethard Mattanovich; KTH; []
    Nyckelord :protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

    Sammanfattning : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. LÄS MER

  3. 2. Human Aquaporins: Production, Characterization and Interactions

    Författare :Jennie Sjöhamn; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporins; membrane protein; protein production; protein characterization; protein:protein interactions; structural biochemistry;

    Sammanfattning : Membrane proteins are essential components of the cell and responsible for the communication with the outside environment and transport of molecules across the membrane. Water transport is facilitated by aquaporins, which are water selective transmembrane pores that serve to maintain cell homeostasis. LÄS MER

  4. 3. Enhancing membrane and secretory protein production yields in Escherichia coli

    Författare :Zhe Zhang; Jan-Willem de Gier; Claes von Wachenfeldt; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; recombinant protein production; Escherichia coli; membrane protein; secretory protein; strain engineering; Biochemistry; biokemi;

    Sammanfattning : Proteins fulfill essential functions in living cells. To produce sufficient amounts of a protein is essential to study the structure and function of a protein, or to use it for medical purposes. Escherichia coli is a Gram-negative bacterium that is widely used for recombinant protein production. LÄS MER

  5. 4. Protein production, characterization and structure determination in structural genomics

    Författare :Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

    Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER

  7. 5. Recombinant subunit vaccines : protein immunogens

    Författare :Sissela Liljeqvist; KTH; []
    Nyckelord :;

    Sammanfattning : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. LÄS MER