Sökning: "affinity proteomics"

Visar resultat 1 - 5 av 66 avhandlingar innehållade orden affinity proteomics.

  1. 1. Functional proteomics : Generation and analysis of cDNA-encoded proteins

    Författare :Susanne Gräslund; KTH; []
    Nyckelord :functional genomics; functional proteomics; gene characterization; E. coli expression; affinity purification; immunolocalization; mouse testis; spermatogenesis; rabbit antibodies; affibody; tektin; Anaphase-promoting complex or cyclosome.;

    Sammanfattning : .... LÄS MER

  3. 2. Global Proteome Survey -Transforming antibody-based affinity proteomics into a global discovery platform

    Författare :Niclas Olsson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity proteomics; recombinant antibodies; Proteomics; mass spectrometry; proteome profiling; breast cancer; antibody specificity;

    Sammanfattning : Proteomics is expected to generate new insights into biological processes as well as identify novel biomarkers and therapeutic targets since most biological functions are transmitted through proteins. However, due to the complexity displayed by a proteome and inherent limitations associated with current methodologies, proteomic analyses often result in incomplete coverage and inconsistent measurements. LÄS MER

  4. 3. Selection of antigens for antibody-based proteomics

    Författare :Lisa Berglund; Mathias Uhlén; Sören Brunak; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; Bioengineering; Bioteknik;

    Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER

  5. 4. Biomarker Discovery in Cancer using Affinity Proteomics - Studies of B-cell lymphomas and Breast Cancer

    Författare :Frida Pauly; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proteomics; affinity proteomics; antibody microarray; cancer; B-cell lymphoma; breast cancer; biomarker discovery;

    Sammanfattning : The continual increase in incidence of cancer has created an urgent need for improved methods for diagnosis, prognosis and/or treatment. In this context, the field of biomarker research has expanded immensely during the last few years. This thesis, based on four original papers, revolves around protein biomarker discovery in cancer. LÄS MER

  7. 5. Affinity assays for profiling disease-associated proteins in human plasma

    Författare :Sanna Byström; Jochen M. Schwenk; Claudia Fredolini; Peter Nilsson; Jennifer Van Eyk; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; affinity proteomics; immunoassay; antibody microarray; suspension bead array; protein profiling; immuno-capture; plasma; serum; cerebrospinal fluid; biomarker discovery; multiple sclerosis; malignant melanoma; mammographic breast density; Bioteknologi; Biotechnology;

    Sammanfattning : Affinity-based proteomics offers opportunities for the discovery and validation of disease-associated proteins in human body fluids. This thesis describes the use of antibody-based immunoassays for multiplexed analysis of proteins in human plasma, serum and cerebrospinal fluid (CSF). LÄS MER