Sökning: "aqueous two phase partitioning"

Visar resultat 11 - 15 av 26 avhandlingar innehållade orden aqueous two phase partitioning.

1. 11. Protein-Polymer Interaction and Association in Aqueous Solution

   Författare :Malin Jönsson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; Fysikalisk kemi; Makromolekylär kemi; Macromolecular chemistry; enzymologi; Proteiner; enzymology; Proteins; Aqueous two-phase systems; Monte Carlo Simulations; hydrophobic effect; surface hydrophobicity; plasmid DNA; Protein; polymer;

   Sammanfattning : The aim of this thesis has been to study protein-polymer interaction and association. Two different techniques were used; Monte Carlo simulations were combined with partitioning in polymeric aqueous two-phase systems. Monte Carlo simulations have been used to evaluate the hydrophobic interaction in electrolyte solution. LÄS MER

3. 12. Influence of Escherichia coli feedstock properties on the performance of primary protein purification

   Författare :Mattias Råvik; Andres Veide; Åsa Emmer; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Downstream processing; cell surface properties; surface plasmon resonance; contact angle measurements; microelectrophoresis; aqueous two-phase partitioning; filtration; EBA adsorbents; high-pressure homogenisation; Biochemistry; Biokemi;

   Sammanfattning : Abstract The aim of the present study was to increase the understanding of how the cell surface properties affect the performance of unit operations used in primary protein purification. In particular, the purpose was to develop, set up and apply methods for studies of cell surface properties and cell interactions. LÄS MER

4. 13. Surface Properties of Antibodies and their Complexes with Antigens Studied by LLPC

   Författare :Christer Wingren; Institutionen för immunteknologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ligand-binding.; surface properties; isomerism; conformational changes; antibody; specificity; two-phase partitioning; antigen-binding sites; Biochemistry; Metabolism; Biokemi; metabolism;

   Sammanfattning : The main objective of this thesis was to gain further insight into the relationship between the exposed surfaces and the functional properties of antibodies and their complexes with antigens in solution. To study this relationship, a new technique, liquid-liquid partition chromatography (LLPC) in aqueous two-phase systems, was developed further with respect to sensitivity, selectivity and reproducibility. LÄS MER

5. 14. Asexual embryogenesis in plants

   Författare :Boel Mårtensson; Biologiska institutionen; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Systematic botany; two-phase partitioning; somatic embryogenesis; pollen embryogenesis; plasma membrane; peroxidase; Daucus carota isoperoxidase; Nicotiana tabacum; Ca2 transport; cell shape; calmodulin; taxonomy; morphology; phytogeography; chemotaxonomy. Physiology of nonvascular plants; Systematisk botanik; taxonomi; morfologi; kemotaxonomi. Växters fysiologi inte kärlväxter .;

   Sammanfattning : The capacity of somatic cells and pollen grains or microspores to undergo embryogenesis is an important property used in improvement and regeneration of plants. Furthermore, somatic embryogenesis as well as pollen embryogenesis show many similarities with zygotic embryogenesis and are, thus, good model systems. LÄS MER

7. 15. Macromolecular interactions, Consequences on carbohydrate metabolism

   Författare :Sandra Matic; Biologiska institutionen; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; macromolecular interactions; sucrose synthase; macromolecular crowding; cytoskeleton; BY-2 tobacco cells; alamethicin; aqueous polymer two-phase partitioning;

   Sammanfattning : Most cells are crowded with solutes, enzymes, nucleic acids, structural proteins and membranes. Crowding promotes macromolecular interactions and determines major properties of the cellular environment such as viscosity, diffusion and inhomogeneity. LÄS MER