Sökning: "aqueous two phase partitioning"

Visar resultat 6 - 10 av 26 avhandlingar innehållade orden aqueous two phase partitioning.

  1. 6. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

  3. 7. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Författare :Cynthia Hassinen; KTH; []
    Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

  4. 8. Selective extraction systems for detection and purification of membrane proteins

    Författare :Ulf Sivars; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Sammanfattning : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. LÄS MER

  5. 9. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  7. 10. Aqueous Block Copolymer Systems. Self-association, Phase Behaviour and Partitioning of Biomolecules

    Författare :Mårten Svensson; Fysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; block copolymer self-association partitioning phase behaviour; Physical chemistry; Fysikalisk kemi;

    Sammanfattning : The phase and partitioning behaviour of aqueous block copolymer systems has been studied. The block copolymers used in this work were built up of three blocks. The middle block was poly(propylene oxide) (PPO) and hydrophobic. The two outer blocks constisted of poly(ethylene oxide) (PEO) chains and less hydrophobic. LÄS MER