Sökning: "enzymology"

Visar resultat 1 - 5 av 74 avhandlingar innehållade ordet enzymology.

1. 1. Exotic Ribosomal Enzymology

   Författare :Josefine Liljeruhm; Anthony C. Forster; Christophe Danelon; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; rRNA modification; Methyltransferase; RlmM; RlmJ; D-amino acid; Unnatural amino acid; Chromoprotein; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : This thesis clarifies intriguing enzymology of the ribosome, the multiRNA/multiprotein complex that catalyzes protein synthesis (translation). The large ribosomal RNAs (23S and 16S rRNAs in E. coli) are post-transcriptionally modified by many specific modification enzymes, yet the functions of the modifications remain enigmatic. LÄS MER

3. 2. β-Mannoside hydrolases from clan A – Structural enzymology, subsite engineering and transglycosylation

   Författare :Anna Rosengren; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannosidase; β-mannanase; enzymology; kinetics; MALDI-TOF MS; protein engineering; protein structure; subsite organisation; transglycosylation;

   Sammanfattning : β-Mannoside hydrolases are enzymes that are involved in conversion of the major soft-wood hemicellulose O-acetyl-galactoglucomannan, an important renewable biomass resource. They belong to clan A of glycoside hydrolases and share a common (β/α)8-TIM barrel fold. LÄS MER

4. 3. The digestive machinery of a human gut bacterium : Structural enzymology of galactomannan utilisation

   Författare :Viktoria Bågenholm; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; Glycoside hydrolase; enzymology; structural biology; kinetics; HPLC; product profiles; structure-function;

   Sammanfattning : Human gut bacteria utilise different types of polysaccharides present in our diet. One of these polysaccharides is galactomannan. Many organisms in the phylum Bacteroidetes have gene clusters encoding for all proteins required for hydrolysis, binding and transport of one type of polysaccharide, called polysaccharide utilisation loci (PULs). LÄS MER

5. 4. Structure and Function of Beta-Mannanases from Glycoside Hydrolase Clan A. A Study of Hemicellulases from Microbes and a Mollusc

   Författare :Lars Anderson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; Proteins; subsite organisation; transglycosylation; enzyme kinetics; beta-mannanase; hemicellulose;

   Sammanfattning : Hemicellulose is the second most abundant group of renewable polysaccharides. Mannans can be found as the major hemicellulose in softwood and as storage polysaccharides in a range of plants. In this work, structural and functional characterisations of family 5 and 26 endo-1,4-b-mannanases (b-mannanases) have been performed. LÄS MER

7. 5. Hemicelluloses from Agricultural and Forestry Crops Isolation, Characterisation, and Enzymatic Hydrolysis

   Författare :Alexandra Andersson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; Physical chemistry; Fysikalisk kemi; enzymology; hydrogels; Plant biochemistry; Växtbiokemi; Proteins; a-galactosidase; b-mannanase; hemicellulase; corn fibre; barley husks; spruce; size-exclusion chromatography; arabinoxylan; microwave irradiation; steam pretreatment; membrane filtration; hemicellulose; galactoglucomannan;

   Sammanfattning : Hemicelluloses are tremendous sources of renewable raw materials, which utilisation have the potential to be highly increased in the future. Hemicelluloses are among the major groups of compounds in plant cell walls, together with cellulose and lignin. These three components are closely associated, making them difficult to separate from each other. LÄS MER