Sökning: "agarose gel electrophoresis"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden agarose gel electrophoresis.

  1. 1. True Monoliths as Separation Media : Homogeneous Gels for Electrophoresis and Electrochromatography in the Capillary and Microchip Modes

    Författare :Ákos Végvári; Douglas Westerlund; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; capillary electrophoresis; capillary electrochromatography; gel electrophoresis; agarose; polyacrylamide; b-cyclodextrin; DNA; peptide; glutathione; drug enantiomers; microchip; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The thesis focuses on the development of new homogeneous gels for the separation of drug enantiomers, peptides, DNA and virus by electrophoresis and electrochromatography in capillaries and microchips. This type of separation media offers high resolution and small zone broadening. LÄS MER

  3. 2. Generic Properties of Polymer Solutions

    Författare :Takashi Uematsu; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; photon correlation spectroscopy; collective diffusion; atomic force microscopy; relaxation; agarose gel electrophoresis; DNA; Zimm dynamics; pulsed-field gradient NMR; superdiffusion; polymer solution; blob model; gel;

    Sammanfattning : Polymer solutions are abundantly present in our daily life, for example in paints, jellies, contact lenses and even in the living cells. Despite their ubiquity, we still have a poor understanding of the physical properties of solutions due to the extremely large number of internal degrees of freedom. LÄS MER

  4. 3. Some Characteristics of Human Prostasomes and Their Relationship to Prostate Cancer

    Författare :Göran Ronquist; Anders Larsson; Lena Carlsson; Alicia Llorente; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Prostasomes; prostate; prostate cancer; clusterin; antibodies; metastasis; exosomes; PC-3 cells; DNA vehicles; gene therapy; immunoblotting; mass spectrometry; agarose gel electrophoresis; vertebral metastasis.; Clinical chemistry; Klinisk kemi; Clinical Chemistry; klinisk kemi;

    Sammanfattning : Background: The secretory epithelial cells of the prostate gland use sophisticated vehicles named prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. LÄS MER

  5. 4. DNA Electrophoresis: Optical Probing and Microscopic Imaging to Understand Separation Mechanisms

    Författare :Anette Larsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; optical probing; YOYO; polarized light spectroscopy; DNA electrophoresis; DAPI; binding modes; helix alignment; fluorescence microscopy;

    Sammanfattning : The aim of this work was to combine spectroscopic, mobility, and fluorescence microscopy studies to elucidate the mechanisms behind DNA electrophoresis, a method to separate DNA molecules according to size. The microscopy studies require staining of the DNA with a fluorescent dye. LÄS MER

  7. 5. Measurement of primary DNA damage in individual cells from humans and mice using the alkaline single cell gel electrophoresis assay

    Författare :Hamid Vaghef; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical biosciences; Farmaceutisk biovetenskap; Biopharmacy; Biofarmaci; arbets- och miljömedicin; Occupational and Environmental Medicine;

    Sammanfattning : Primary DNA damage is considered to be an important step in the development of tumours and itmay also serve as a biomarker of genotoxic stress. Measurement of DNA strand breaks in peripherallymphocytes can yield useful information about the suitability of these cells for biomonitoring purposesand subsequently risk assessment. LÄS MER