Sökning: "immunoblotting"

Visar resultat 1 - 5 av 70 avhandlingar innehållade ordet immunoblotting.

  1. 1. Helicobacter pylori cell surface interactions with glycosaminoglycans. Identification and characterisation of proteins binding to heparin/heparan sulphate

    Författare :Meeme Utt; Celiaki och diabetes; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; SELDI-TOF MS; bioinformatics; proteomics; immunoblotting; 2-DE; biosensor; heparan sulphate; heparin; binding; Helicobacter pylori; cell surface; bakteriologi; Mikrobiologi; mycology; bacteriology; Microbiology; mykologi; virologi; virology;

    Sammanfattning : Helicobacter pylori is a gastric pathogen which cause chronic type B gastritis and peptic ulcer disease. H. pylori produces virulence factors such as urease, vacuolating cytotoxin VacA, cag pathogenicity island-associated proteins, flagella and adhesins. LÄS MER

  3. 2. The Biodiversity of Hydrogenases in Frankia : Characterization, regulation and phylogeny

    Författare :Melakeselam Leul Zerihun; Anita Sellstedt; Kornel Kovács; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biodiversity; Frankia; immunoblotting; gene expression; uptake hydrogenase; hydrogen-evolving hydrogenase; nickel; phylogeny; Molecular biology; Molekylärbiologi;

    Sammanfattning : All the eighteen Frankia strains isolated from ten different actinorhizal host plants showed uptake hydrogenase activity. The activity of this enzyme is further increased by addition of nickel. Nickel also enhanced the degree of hydrogenase transfer into the membranes of Frankia, indicating the role of this metal in the processing of this enzyme. LÄS MER

  4. 3. Some Characteristics of Human Prostasomes and Their Relationship to Prostate Cancer

    Författare :Göran Ronquist; Anders Larsson; Lena Carlsson; Alicia Llorente; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Prostasomes; prostate; prostate cancer; clusterin; antibodies; metastasis; exosomes; PC-3 cells; DNA vehicles; gene therapy; immunoblotting; mass spectrometry; agarose gel electrophoresis; vertebral metastasis.; Clinical chemistry; Klinisk kemi; Clinical Chemistry; klinisk kemi;

    Sammanfattning : Background: The secretory epithelial cells of the prostate gland use sophisticated vehicles named prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. LÄS MER

  5. 4. Studies of immune responses to cell surface proteins of Helicobacter pylori and Borrelia burgdorferi by enzyme immunoassay and immunoblotting

    Författare :Ingrid Nilsson; Avdelningen för medicinsk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mikrobiologi; mycology; virology; B. burgdorferi; H.pylori; cell surface proteins; gel-electrophoresis; enzyme immunoassay; Western immunoblotting; bacteriology; Microbiology; bakteriologi; virologi; mykologi;

    Sammanfattning : Helicobacter pylori and Borrelia burgdorferi s.l. can cause chronic infections by evading the immune system. These two human pathogens express a number of immunogenic cell surface proteins. LÄS MER

  7. 5. The Impact of Drugs of Abuse on the Neuroproteome : Application of Immunoblotting and LC-MS-based Proteomics

    Författare :Uwe Rossbach; Pierre Le Grevès; Fred Nyberg; Matthias Hallberg; Jan Kehr; Uppsala universitet; []
    Nyckelord :;

    Sammanfattning : Qualitative and quantitative analysis of proteins can be based either on immunological or on mass spectrometry-utilizing methods. In this thesis, different approaches of protein analysis were applied to elucidate the impact of drugs of abuse on the rat brain. Androgens have been reported to affect mood, anxiety and memory function. LÄS MER