Sökning: "MALDI-MS"

Visar resultat 1 - 5 av 35 avhandlingar innehållade ordet MALDI-MS.

  1. 1. Integral membrane protein MALDI-MS analysis - Sample handling and fabrication of a structured target

    Författare :Theres Redeby; KTH; []
    Nyckelord :Integral membrane proteins; Hydrophobic peptides; Sample handling; MALDI-MS; Structured sample support;

    Sammanfattning : .... LÄS MER

  3. 2. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

    Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER

  4. 3. Nucleic Acids as Drugs and Drug Targets With Focus on Anticancer Active Platinum Complexes and Small Interfering RNAs

    Författare :Hanna Hedman; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; siRNA; thermodynamic stability; mass spectrometry; MALDI-MS; chemical probing; luciferase assay;

    Sammanfattning : To ensure that patients receive a safe and effective treatment, it is important to understand how drugs interact with and affect molecules within a cell. Here, evaluation of platinum based drugs and their ability to interfere with nucleic acid functions is presented. LÄS MER

  5. 4. Strategies for Miniaturized Biomarker Detection

    Författare :Belinda Adler; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

    Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER

  7. 5. Development and application of alternative methods for profiling proteins N-glycosylation

    Författare :Alessandro Quaranta; Leopold Ilag; Niclas Karlsson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; N-glycosylation; Glycomics; Glycosylation Biomarkers; Intact Glycoproteins; Glycoform Quantification; Mass Spectrometry; Ion Mobility Spectrometry; MALDI-MS; Paper Spray Ionization; Microfluidics; Magnetic Beads; Immunoaffinity Purification; Nanobodies; Analytical Chemistry; analytisk kemi;

    Sammanfattning : Glycosylation is a post-translational modification (PTM) that exerts profound structural and functional effects on the modified protein. Glycan synthesis and conjugation to proteins are regulated by a myriad of factors, both genetic and environmental, and are also influenced by external stressors. LÄS MER