Sökning: "siRNA"

Visar resultat 1 - 5 av 182 avhandlingar innehållade ordet siRNA.

1. 1. miRNA and Asymmetric siRNA : Small RNAs with Large Effects on Bone Metabolism

   Författare :Navya Laxman; Andreas Kindmark; Sara Windahl; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; miRNA; cp-siRNA; RNAi; bone; Osteoblast; Sequencing; Differential expression; Wnt pathway; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : RNA interference (RNAi) is a post-transcriptional gene silencing process elicited by double-stranded RNA, such as micro-RNA (miRNA) and small interfering RNA (siRNA). They are 18-25 nucleotide long, small non-coding RNAs acting as critical regulators in eukaryotic genome expression. LÄS MER

3. 2. Nucleic Acids as Drug Targets -Interactions of Platinum Complexes, Polyamines, and siRNA with DNA and RNA Models

   Författare :Margareta Hägerlöf; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kinetics; Kemi; Chemistry; AU-rich region; siRNA; Circular Dichroism; Polyamine; Modified Nucleoside; HPLC; Thermodynamic Stability; cisplatin; Hairpin; DNA and RNA conformation; RNA; DNA; Anticancer Drug; Platinum Complexes;

   Sammanfattning : Drugs targeting nucleic acids were studied in DNA and RNA model systems. The effects were investigated with respect to structural and thermodynamic influence, kinetics, and biological function. The drugs employed in this thesis were anticancer active platinum(II) complexes, polyamine derivatives, and siRNA. LÄS MER

4. 3. Linear and Branched Chitosan Oligomers as Delivery Systems for pDNA and siRNA In Vitro and In Vivo

   Författare :Mohamed Mahmoud Issa; Per Artursson; Arto Urtti; Uppsala universitet; []
   Nyckelord :Medical sciences; Chitosan oligomers; gene delivery; gene expression; pDNA; siRNA; MEDICIN OCH VÅRD;

   Sammanfattning : In this thesis, chitosan, a biocompatible polysaccharide that has been approved as a food additive was selected as a platform for the development of safe, efficient non-viral gene delivery systems to mammalian cells. Previously, chitosan-based gene formulations had been generally associated with high molecular weight chitosans, which were poorly characterised in terms of molecular weight distribution and degree of acetylation. LÄS MER

5. 4. Nucleic Acids as Drugs and Drug Targets With Focus on Anticancer Active Platinum Complexes and Small Interfering RNAs

   Författare :Hanna Hedman; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; siRNA; thermodynamic stability; mass spectrometry; MALDI-MS; chemical probing; luciferase assay;

   Sammanfattning : To ensure that patients receive a safe and effective treatment, it is important to understand how drugs interact with and affect molecules within a cell. Here, evaluation of platinum based drugs and their ability to interfere with nucleic acid functions is presented. LÄS MER

7. 5. Chimeric gene delivery vectors : Design, synthesis, and mechanisms from transcriptomics analysis

   Författare :Moataz Dowaidar; Ülo Langel; Sandrine Sagan; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptides; magnetic nanoparticles; graphene oxide; autophagy; siRNA; SCO; QSAR; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

   Sammanfattning : Delivery of nucleic acid is a promising approach for genetic diseases/disorders. However, gene therapy using oligonucleotides (ONs) suffers from low transfection efficacy due to negative charges, weak cellular permeability, and enzymatic degradation. LÄS MER