Sökning: "Protein cleavage digestion."

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden Protein cleavage digestion..

1. 1. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

   Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER

3. 2. Mass spectrometry in protein structure analysis

   Författare :Andreas Jonsson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Mass spectrometry is an important analytical tool in biological and biochemical research. The speed, accuracy and sensitivity is unmatched by conventional analytical techniques. LÄS MER

4. 3. Chemical and mass spectrometrical methods in protein analysis

   Författare :Madalina Oppermann; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Sensitive analytical methods are essential in protein studies because of frequently encountered limited sample availability. There is a constant need for optimization of sample preparation and analytical procedures for structural characterization of biomedically relevant proteins. Amino acid sequences can be derived from genomic data. LÄS MER

5. 4. Intracellular systems for characterization and engineering of proteases and their substrates

   Författare :George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. LÄS MER

7. 5. Investigating mucin interactions with diverse surfaces for biomedical applications

   Författare :Georgia Petrou; Thomas Crouzier; Anette Larsson; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; mucus; mucin; protein adsorption; biomaterials; lubrication; mucoadhesion; 4RepCT; Bioteknologi; Biotechnology;

   Sammanfattning : Mucous membranes are covered with mucus, a viscoelastic hydrogel that plays an essential role in their protection from shear and pathogens. The viscoelasticity of mucus is owing to mucins, a group of densely glycosylated proteins. LÄS MER