Sökning: "Glycosylation Biomarkers"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden Glycosylation Biomarkers.

  1. 1. Development and application of alternative methods for profiling proteins N-glycosylation

    Författare :Alessandro Quaranta; Leopold Ilag; Niclas Karlsson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; N-glycosylation; Glycomics; Glycosylation Biomarkers; Intact Glycoproteins; Glycoform Quantification; Mass Spectrometry; Ion Mobility Spectrometry; MALDI-MS; Paper Spray Ionization; Microfluidics; Magnetic Beads; Immunoaffinity Purification; Nanobodies; Analytical Chemistry; analytisk kemi;

    Sammanfattning : Glycosylation is a post-translational modification (PTM) that exerts profound structural and functional effects on the modified protein. Glycan synthesis and conjugation to proteins are regulated by a myriad of factors, both genetic and environmental, and are also influenced by external stressors. LÄS MER

  3. 2. Development and Application of Proximity Assays for Proteome Analysis in Medicine

    Författare :Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

    Sammanfattning : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. LÄS MER

  4. 3. Development of proteomic methods for studying cerebrospinal fluid proteins involved in Alzheimer´s disease

    Författare :Maja Puchades; Göteborgs universitet; []
    Nyckelord :Alzheimer s disease; cerebrospinal fluid; proteomics; mass spectrometry; biomarkers; synaptic proteins; liquid phase IEF; biomarkers;

    Sammanfattning : Alzheimer's disease (AD) is the most common cause of dementia in western countries. The main neuropathological findings in the AD brain are senile plaques, neurofibrillary tangles and degeneration of neurons and synapses. LÄS MER

  5. 4. Targeting the human glycoproteome: new enrichment protocols and mass spectrometric analyses reveal unique and novel glycosylation sites

    Författare :Adnan Halim; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Glycobiology; Glycoproteomics; Mass Spectrometry;

    Sammanfattning : Glycosylation is one of the most common and structurally diverse posttranslational modifications of proteins. Given that protein glycosylation is involved in various cellular processes, the characterization of site-specific N- and O-linked glycosylations is becoming increasingly important. However, current mass spectrometry-based technologies, i.e. LÄS MER

  7. 5. Advances and Applications of Antibody Arrays - Proteomic Profiling of Pancreatic Disease

    Författare :Anna Sandström Gerdtsson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proteomics; affinity proteomics; antibody microarray; pancreatic cancer; pancreatitis; biomarker; immunosignature;

    Sammanfattning : Recombinant antibody microarrays have advanced into indispensable tools for large-scale, high-throughput multiplexed serum proteomics. This thesis, based upon five original papers, deals with the development of an in-house designed antibody microarray platform, and its applications for serum profiling of pancreatic disease. LÄS MER