Sökning: "Matrix-Assisted Laser Desorption Ionization Mass Spectrometry"

Visar resultat 1 - 5 av 49 avhandlingar innehållade orden Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.

1. 1. Electrophoretic focusing in microchannels combined with mass spectrometry : Applications on amyloid beta peptides

   Författare :Saara Mikkonen; Åsa Emmer; Johan Jacksén; Tapio Kotiaho; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; amyloid beta; computer simulation; electrophoresis; electrospray ionization; isoelectric focusing; isotachophoresis; MALDI; mass spectrometry; microchannel; microchip; nano-electrospray ionization; preconcentration; separation; Kemi; Chemistry;

   Sammanfattning : Analysis of low-abundance components in small samples remains a challenge within bioanalytical chemistry, and new techniques for sample pretreatments followed by sensitive and informative detection are required. In this thesis, procedures for preconcentration and separation of proteins and peptides in open microchannels fabricated on silicon microchips are presented. LÄS MER

3. 2. Label free analysis and spatial distribution of molecular lipids in brain tissue and cells using mass spectrometry imaging

   Författare :Amir Saeid Mohammadi; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Mass Spectrometry Imaging; Secondary Ion Mass Spectrometry Imaging; Matrix Assisted Laser desorption Ionization; Lipids; Brain tissue;

   Sammanfattning : Lipids are important inherent components in brain and cell membranes, which play significant biological roles in cell function. Identification and localization of specific biomolecules such as peptides and lipids within cellular membranes is currently a major challenge in metabolomics and biological studies. LÄS MER

4. 3. Tools for applied soft ionization mass spectrometry

   Författare :Joakim Romson; Åsa Emmer; Peter Dinér; Steven Wilson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; Electrospray ionization; Matrix assisted laser desorption ionization; Mass accuracy; Mass shifts; Ion trap; Capillary electrophoresis; Sample concentration; Masspektrometri; Elektrospray-jonisering; Matrisassisterad laser desorbtion jonisering; Mass-exakthet; Masskift; Jonfälla; Kapillärelektrofores; Provkoncentration; Kemi; Chemistry;

   Sammanfattning :  Mass spectrometry (MS) is an important analytical tool. Its most importantadvantages are sensitivity, resolution, and accurate and precise m/zmeasurements. To get the best results from an instrument, the user musthave knowledge of its limitation and requirements. LÄS MER

5. 4. Sample preconcentration in open microchannels : Combinations with MALDI and nano-ESI mass spectrometry and computer simulations

   Författare :Saara Mikkonen; Åsa Emmer; Leopold Ilag; Jenny Ottosson Takanen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Preconcentration; open microchannel; microchip; MALDI; electrospray ionization; nanoelectrospray; mass spectrometry; computer simulation;

   Sammanfattning : In this thesis a novel concept for preconcentration of biomolecules in open microchannels is presented. The preconcentration is based on electromigration of charged analytes, and detection is performed with matrix-assisted laser desorption/ionization (MALDI) or nano-electrospray ionization (nESI) mass spectrometry (MS). LÄS MER

7. 5. Development of Methods for Protein and Peptide Analysis Applied in Neuroscience Utilizing Mass Spectrometry

   Författare :Johan Pierson; Per Andrén; Ulf Bondesson; György Marko-Varga; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Mass spectrometry; Parkinson’s disease; Metabolism; Profiling Mass Spectrometry; Proteomics; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : This thesis describes the utilization of the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) and electrospray ionization (ESI) MS techniques for analysis of complex brain tissue samples. Direct molecular profiling of biological samples using MALDI MS is a powerful tool for identifying phenotypic markers. LÄS MER