Sökning: "Competitive assays"

Visar resultat 1 - 5 av 33 avhandlingar innehållade orden Competitive assays.

  1. 1. Strategies for developing peptide and protein selective plastic antibodies

    Författare :Rashmi Mahajan; Ian A. Nicholls; Subramanian Suriyanarayanan; Börje Sellergren; Alessandra Maria Bossi; Linnéuniversitetet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Competitive assays; molecularly imprinted polymer; magnetic nanoparticle; nanogel; nanoparticle; plastic antibody; solid-phase synthesis; QCM.; Biokemi; Biochemistry;

    Sammanfattning : The objective of the thesis was to explore approaches for the development of molecularly imprinted materials with predetermined selectivity for peptide or protein targets. In particular, the work has focused on molecularly imprinted nanoparticles synthesized using solid-phase synthesis protocols. LÄS MER

  3. 2. Competitive-Binding Based Optical DNA Mapping - From Bacterial Plasmids to the Human Genome

    Författare :Vilhelm Müller; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genetics; single-molecule; nanofluidics; DNA; antibiotic resistance; optical DNA mapping; plasmids; competitive binding; fluorescence microscopy; bacteria;

    Sammanfattning : Significant advances within the field of DNA sequencing have allowed us to study DNA at a level of detail that was previously impossible. However, dynamic genomic regions with a high degree of structural variations, while being linked to disease in humans and increased resistance to antibiotics, are still challenging to characterize. LÄS MER

  4. 3. Genetic Sequence Analysis by Microarray Technology

    Författare :Emilie Hultin; Joakim Lundeberg; Afshin Ahmadian; Marie Allen; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genotyping; single nucleotide polymorphism SNP ; protease-mediated allele-specific extension PrASE ; microarray; tag-array; competitive hybridization; human papillomavirus HPV ; single cell; loss of alleles; differentiation; epidermis.; Bioengineering; Bioteknik;

    Sammanfattning : Developments within the field of genetic analysis have during the last decade become enormous. Advances in DNA sequencing technology have increased throughput from a thousand bases to over a billion bases in a day and decreased the cost thousandfold per base. LÄS MER

  5. 4. Magnetic Nanoparticle Based Biosensors for Pathogen Detection and Cancer Diagnostics

    Författare :Bo Tian; Mattias Strömberg; Peter Svedlindh; Hakho Lee; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Magnetic biosensors; magnetic nanoparticles; homogeneous assays; volumetric sensing; Engineering Science with specialization in Solid State Physics; Teknisk fysik med inriktning mot fasta tillståndets fysik;

    Sammanfattning : This thesis describes several magnetic nanoparticle (MNP)-based biosensing strategies which take advantage of different magnetic sensors, molecular tools and nanotechnologies. Proposed biosensors can be classified into three groups, i.e., immunoassay-based, molecular amplification-based, and nanoparticle assembly-based. LÄS MER

  7. 5. Fluorescence-based ligand assays for protein detection using affibody affinity proteins

    Författare :Björn Renberg; Amelie Eriksson Karlström; Per-Åke Nygren; Jörg Hoheisel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; biosensors; FRET; immobilisation; solid-phase synthesis; protein microarrays; Biochemistry; Biokemi;

    Sammanfattning : The detection and quantification of biomolecules, and proteins in particular, are of great interest since these molecules are of fundamental importance to our well-being. Body fluids, as for instance human blood, are well suited for sampling of protein levels. LÄS MER