Sökning: "FRET"

Visar resultat 1 - 5 av 67 avhandlingar innehållade ordet FRET.

  1. 1. DNA and RNA base analogue FRET - from fluorophore design to biochemical applications

    Författare :Moa Sandberg Wranne; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; adenine analogue; Fluorescent base analogue; FRET; thymine analogue; netropsin; fluorescence; DNA; RNA; Z-RNA;

    Sammanfattning : This thesis focuses on the development and use of fluorescent base analogues (FBAs). They are important tools in research concerning nucleic acids structure, dynamics and interactions. LÄS MER

  2. 2. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

    Författare :Evangelos Sisamakis; Jerker Widengren; Paul R Selvin; KTH; []

    Sammanfattning : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. LÄS MER

  3. 3. Development of Fluorescent Nucleobase Analogues - Intrinsically labelled nucleic acids for molecular binding investigations

    Författare :Mattias Bood; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; Fluorescent nucleobase analogue; FRET; pre-microRNA; isothermal titration calorimetry; surface plasmon resonance; aminoglycoside; binding interaction;

    Sammanfattning : This thesis focuses on the design, synthesis and utilization of fluorescent nucleobase analogues (FBAs). FBAs are an important class of compounds, used in the research of nucleic acids. The class of canonical FBAs, i.e. LÄS MER

  4. 4. Fluorescence-based ligand assays for protein detection using affibody affinity proteins

    Författare :Björn Renberg; Amelie Eriksson Karlström; Per-Åke Nygren; Jörg Hoheisel; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; biosensors; FRET; immobilisation; solid-phase synthesis; protein microarrays; Biochemistry; Biokemi;

    Sammanfattning : The detection and quantification of biomolecules, and proteins in particular, are of great interest since these molecules are of fundamental importance to our well-being. Body fluids, as for instance human blood, are well suited for sampling of protein levels. LÄS MER

  5. 5. Adsorption of biopolymers and their layer-by-layer assemblies on hydrophilic surfaces

    Författare :Maria Lundin; Eva Blomberg; Thomas Arnebrant; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Layer-by-layer; multilayer; adsorption; biopolymers; Chitosan; Heparin; Mucin; Albumin; Lysozyme; β-casein; SDS; QCM-D; Ellipsometri; DPI; TIRF; FRET; SFA; layer structure; solvent content; vertical diffusion; exponential growth; solid-liquid interface; deposition conditions; Surface and colloid chemistry; Yt- och kolloidkemi;

    Sammanfattning : It is widely known that surfaces play an important role in numerous biological processes and technological applications. Thus, being able to modify surface properties provides an opportunity to control many phenomena occurring at interfaces. LÄS MER