Sökning: "Capillary electrophoresis CE"

Visar resultat 16 - 20 av 45 avhandlingar innehållade orden Capillary electrophoresis CE.

1. 16. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

   Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER

3. 17. Spatial metabolomics using Surface Sampling Capillary Electrophoresis Mass Spectrometry  : New tool for direct surface sampling chemical analysis of biological samples

   Författare :Anastasia Golubova; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Analytical surface sampling tools enable direct chemical studies of solid biological samples, including morphologically diverse tissue, and provide both fast chemical analysis with minimum sample preparation and information about molecular spatial distribution. Spatially resolved investigations of tissue sections could help to reveal pathological mechanisms at specific tissue locations. LÄS MER

4. 18. Methods for protein analysis by capillary electrophoresis and mass spectrometry

   Författare :Joakim Romson; Åsa Emmer; Peter Dinér; Lukas Käll; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MALDI; Concentration plates; Alkyl ketene dimer; CE-MALDI-MS2; On-target digestion; Automation; Robot; Pieris napi; spermatophore; ESI-MS2; Osteopontin; MALDI; Koncentrationsprovplatta; alkylketenedimer; CE-MALDI-MS2; enzymklyvning på provplatta; Automatisering; Robot; Pieris napi; Spermatofor; ESI-MS2; Osteopontin; Kemi; Chemistry;

   Sammanfattning : Protein analysis is important to understanding biological systems, but sample diversity necessitates a multitude of analysis techniques and methods. Challenges that are addressed include analysis of low abundance samples, fractionation to reduce sample complexity, and automation to reduce time and cost. LÄS MER

5. 19. Development of Methods in CE, CE-MS and MS/MS : Applications in Pharmaceutical, Biomedical and Forensic sciences

   Författare :Emmy Jäverfalk-Hoyes; Jonas Bergquist; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical chemistry; Farmaceutisk kemi; Pharmaceutical chemistry; Farmaceutisk kemi; analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry;

   Sammanfattning : Capillary electrophoresis-mass spectrometry has been used successfully for the analysis of a wide range of analytes such as chiral local anaesthetics, sulphonated reactive dyes and endogenous neurotransmitters and neuropeptides.The partial filling technique was used in CE-MS for chiral separation of bupivacaine and ropivacaine using the non-volatile selector β-cyclodextrin. LÄS MER

7. 20. Analytical Quality by Design Method Development for Vaccine Characterization

   Författare :Lars Geurink; Catharina Sänger van de Griend; Mikael Hedeland; Martijn Schenning; Iulia Oita; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; viral vaccines; adenovirus; influenza; polio; COVID; corona; capillary electrophoresis; analytical quality by design; analytical method development; CGE; icIEF; CZE; crude cell suspension analysis; in-process sample analysis; quality control testing; biopharmaceutical characterization; Analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry;

   Sammanfattning : Vaccines that are safe, efficacious, and can be rapidly developed are needed to prevent and to react to emerging global infectious disease threats such as influenza, Polio, and Coronavirus diseases. Fast and reliable analytical methods are required without delay to support vaccine process and product development, characterization, and quality control testing. LÄS MER