Sökning: "Capillary electrophoresis CE"

Visar resultat 11 - 15 av 45 avhandlingar innehållade orden Capillary electrophoresis CE.

  1. 11. Electrifying the Molecules of Life : Peptide and Protein Analysis by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry

    Författare :Magnus Wetterhall; Jonas Bergquist; Karin E. Markides; Dominic M. Desiderio; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Capillary electrophoresis CE ; Electrospray ionization ESI ; Mass spectrometry MS ; Peptides; Proteins; Cerebrospinal fluid CSF ; Graphite coating; Monomer coating; Polymer coating; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Sammanfattning : This thesis describes the current status and novel aspects of the analysis of the molecules of life, i.e. peptides and proteins, using capillary electrophoresis (CE) coupled to mass spectrometry (MS) via (sheathless) electrospray ionization (ESI). Early reports of sheathless CE-ESI-MS were plagued by limited lifetimes of the electrospray emitter. LÄS MER

  3. 12. Surface Modified Capillaries in Capillary Electrophoresis Coupled to Mass Spectrometry : Method Development and Exploration of the Potential of Capillary Electrophoresis as a Proteomic Tool

    Författare :Aida Zuberovic; Jonas Bergquist; Ziad El Rassi; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Topology optimization; Design optimization; Material distribution; Wave propagation problems; Inverse problems; Acoustic devices; Medical microwave tomography; High performance computing; Analytical chemistry; Analytisk kemi;

    Sammanfattning : The increased knowledge about the complexity of the physiological processes increases the demand on the analytical techniques employed to explore them. A comprehensive analysis of the entire sample content is today the most common approach to investigate the molecular interplay behind a physiological deviation. LÄS MER

  4. 13. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

    Författare :Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. LÄS MER

  5. 14. Capillary Electrophoresis Methodology for Complex Biological Sample Matrices

    Författare :Joanna Olsson; Richard Cassidy; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical Chemistry; analytisk kemi;

    Sammanfattning : This thesis is based on five papers where capillary electrophoresis (CE) is utilised for determination of various analytes in complex biological matrices.... LÄS MER

  7. 15. Capillary electrophoresis mass spectrometry applied to structural proteomics and small molecule analysis

    Författare :Jordan Aerts; Erik T. Jansson; Per E. Andrén; Petr Man; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary Electrophoresis Mass Spectrometry; Hydrogen Deuterium Exchange Mass Spectrometry; Enantioselective Separations; Drugs of Abuse; Analytical Chemistry; Analytisk kemi; Analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

    Sammanfattning : Capillary electrophoresis with mass spectrometric (CE–MS) detection offers a separation method without equal in terms of flexibility, utility, and cost efficiency. Here we demonstrate precisely this through the application of several laboratory-built CE–MS instruments for the separation of brain metabolites in non-primates, enantioselective separations of synthetic anesthetic metabolites in fractionated pony urine, application in structural proteomics workflows, and identification of exogenous alkaloid biotransformationproducts in human cerebrospinal fluid (CSF). LÄS MER