Sökning: "surface display"

Visar resultat 1 - 5 av 176 avhandlingar innehållade orden surface display.

  1. 1. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Biotechnology; Bioteknologi;

    Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER

  2. 2. Staphylococcal surface display for protein engineering and characterization

    Författare :John Löfblom; Stefan Ståhl; Henrik Wernérus; K. Dane Wittrup; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; affibody; combinatorial library; epitope mapping; Gram-positive bacteria; protein engineering; staphylococcal surface display; Molecular biology; Molekylärbiologi;

    Sammanfattning : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. LÄS MER

  3. 3. Interfacial Properties of Biomacromolecular Model Systems: Surface Forces and Nanotribology

    Författare :Niklas Nordgren; Mark Rutland; Harry Brumer III; Robert Pelton; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; afm; surface force; friction; composite material; model surface; quartz crystal microbalance; dissipation; cellulose; xyloglucan; chitosan; colloidal probe; nanotribology; biomimetic; enzymatic; polysaccharide; graft; fiber; thiol; spring constant; calibration; lubrication; polyion; responsive.; Surface and colloid chemistry; Yt- och kolloidkemi;

    Sammanfattning : The nanotribology, adhesion and related interfacial properties of biomacromolecular systems have been studied.  The aim was to elucidate the role of physisorbed and chemically grafted bio-polymers implicated as mediators for cellulose based processing and material design. LÄS MER

  4. 4. Staphylococcal surface display in directed evolution

    Författare :Nina Kronqvist; Stefan Ståhl; Mats A. A. Persson; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; affibody; combinatorial library; directed evoluation; Gram-positive bacteria. protein engineering; Bioengineering; Bioteknik;

    Sammanfattning : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. LÄS MER

  5. 5. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies

    Författare :Ann-Christin Malmborg Hager; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; affinity; antibody; kinetic; Immunologi; serologi; serology; Immunology; Bioteknik; Biotechnology; library; selection; surface display; phage display; association rate; dissociation rate;

    Sammanfattning : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. LÄS MER