Sökning: "surface display"

Visar resultat 6 - 10 av 188 avhandlingar innehållade orden surface display.

  1. 6. Bacterial display systems for engineering of affinity proteins

    Författare :Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
    Nyckelord :Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

    Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER

  3. 7. Perilipin expression in the islets of Langerhans and Surface expression in the baculovirus/insect cell system

    Författare :Jörgen Borg; Molekylär endokrinologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biology; Biologi; lipotoxicity; beta-cell; perilipin; amino-terminal anchor; Baculovirus; surface display;

    Sammanfattning : Surface display in insect cells: Systems available for surface display that utilize bacterial phages are insufficient for displaying a number of eukaryotic proteins. By instead presenting proteins on the surface of eukaryotic cells or virus, some of these problems might be overcome. LÄS MER

  4. 8. Staphylococcal surface display and its applications in bioscience

    Författare :Patrik Samuelson; KTH; []
    Nyckelord :;

    Sammanfattning : Display of heterologous proteins on the surface ofmicroorganisms, enabled by means of recombinant DNA technology,has become an increasingly used strategy in variousapplications in microbiology, biotechnology and vaccinology.This thesis presents two bacterial systems, designed forsurface display of chimeric proteins on the food-gradestaphylococcal speciesStaphylococcus xylosusandStaphylococcus carnosus, respectively. LÄS MER

  5. 9. Functional studies and engineering of family 1 carbohydrate-binding modules

    Författare :Janne Lehtiö; KTH; []
    Nyckelord :Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Sammanfattning : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. LÄS MER

  7. 10. Discovery of targets for tumor immunotherapy using phage display technology

    Författare :Jesper Tordsson; Immunologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; serology; Immunology; colon cancer; melanoma; tumor immunotherapy; phage display; scFv; Immunologi; serologi; Cytology; oncology; cancerology; Cytologi; onkologi; cancer;

    Sammanfattning : This thesis has focused on the development of methods and their applications for the identification of antibodies reactive with tumor associated antigens (TAA). The phage display technology provides a powerful mean to generate antibodies potentially useful for tumor immunotherapy. LÄS MER