Sökning: "Harry Brumer III"

Hittade 4 avhandlingar innehållade orden Harry Brumer III.

  1. 1. Interfacial Properties of Biomacromolecular Model Systems: Surface Forces and Nanotribology

    Författare :Niklas Nordgren; Mark Rutland; Harry Brumer III; Robert Pelton; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; afm; surface force; friction; composite material; model surface; quartz crystal microbalance; dissipation; cellulose; xyloglucan; chitosan; colloidal probe; nanotribology; biomimetic; enzymatic; polysaccharide; graft; fiber; thiol; spring constant; calibration; lubrication; polyion; responsive.; Surface and colloid chemistry; Yt- och kolloidkemi;

    Sammanfattning : The nanotribology, adhesion and related interfacial properties of biomacromolecular systems have been studied.  The aim was to elucidate the role of physisorbed and chemically grafted bio-polymers implicated as mediators for cellulose based processing and material design. LÄS MER

  3. 2. In vitro and in vivo approaches in the characterization of XTH gene products

    Författare :Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. LÄS MER

  4. 3. Enzyme selectivity as a tool in analytical chemistry

    Författare :Anders Hamberg; Karl Hult; Harry Brumer; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; substrate specificity; competing substrates; enantiomeric excess analysis; library screening; ladder sequencing; carboxypeptidase Y; 2-pyridylmethylamine; MALDI; Candia antarctica lipase B; pig liver esterase; horse liver alcohol dehydrogenase; acylated cyanohydrins; Biochemistry; Biokemi;

    Sammanfattning : Enzymes are useful tools as specific analytical reagents. Two different analysis methods were developed for use in the separate fields of protein science and organic synthesis. Both methods rely on the substrate specificity of enzymes. Enzyme catalysis and substrate specificity is described and put in context with each of the two developed methods. LÄS MER

  5. 4. Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

    Författare :Gustav Sundqvist; Harry Brumer; Per Andrén; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; noncovalent complex; droplet fission; nano-electrospray ionisation; mass spectrometry; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, the application of electrospray ionisation mass spectrometry (ESI-MS) to the analysis of intact proteins is demonstrated. In papers I and II, the use of ESI-MS for the analysis of noncovalent protein-ligand complexes were discussed. LÄS MER