Sökning: "sensitive assay"

Visar resultat 16 - 20 av 257 avhandlingar innehållade orden sensitive assay.

1. 16. New sensitive and specific methods for sex steroid measurements in rodent serum and tissues

   Författare :Maria E. Nilsson; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; sex steroids; mass spectrometry; rodents;

   Sammanfattning : Accurate measurement of sex steroids (androgens, estrogens, and progesterone) is essential in research and clinical settings for common diseases including osteoporosis, prostate cancer, breast cancer, and cardiovascular diseases. Sex steroids are not only produced in the gonads and adrenals but may also be locally synthesized in peripheral target tissues. LÄS MER

3. 17. Assay development for studies of G protein-coupled receptors at the single-molecule level

   Författare :Olov Wahlsten; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; colocalization; drug discovery; QCM-D; GPCR; pharmacodynamics; kinetics; equilibrium fluctuation analysis;

   Sammanfattning : G protein-coupled receptors (GPCRs), also known as seven transmembrane (7TM) receptors,is the largest family of cell surface receptors. These receptors play a key role in transmittinga wide variety of signals across the cell membrane and are involved in physiologicalprocesses such as sensory transduction, cell-cell communication, neuronal transmission,and hormonal signaling. LÄS MER

4. 18. Use of affordable technology for the sensitive and specific diagnosis of onchocerciasis (river blindness)

   Författare :Gabriel Eduardo Guzmán Laparra; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Onchocerciasis or river blindness is the most common infectious cause of blindness. Even in the absence of blindness, the skin effects caused are of social stigma. It is the goal of the World Health Organization to eradicate filariasis (including onchocerciasis). LÄS MER

5. 19. Detection and Sequencing of Amplified Single Molecules

   Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

7. 20. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

   Författare :Tonge Ebai; Ulf Landegren; Masood Kamali-Moghaddam; Peter Nilsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

   Sammanfattning : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. LÄS MER