Sökning: "rolling circle amplification"

Visar resultat 1 - 5 av 43 avhandlingar innehållade orden rolling circle amplification.

  1. 1. Padlock Probes and Rolling Circle Amplification : New Possibilities for Sensitive Gene Detection

    Författare :Maritha Mendel-Hartvig; Pål Nyrén; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. LÄS MER

  2. 2. Padlock Probe-Based Nucleic Acid Amplification Tests : Point-of-care Diagnostics of Infectious Diseases

    Författare :Sibel Ciftci; Mats Nilsson; Jonas Klingström; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Nucleic Acid Amplification; Isothermal Amplification Methods; Padlock Probes; Rolling Circle Amplification; Molecular Diagnostics; Infectious Disease Diagnostics; Virus; Point-of-Care; biokemi; Biochemistry;

    Sammanfattning : Recent advancements in molecular biology and biotechnology have pushed the field of molecular diagnostics much further to benefit the society towards smart access for rapid and simplified health care. In this context, point-of-care (PoC) technologies that bring the inventions in diagnostics closer to bedside settings draw attention. LÄS MER

  3. 3. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

    Författare :Johan Banér; Sanjay Tyagi; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

    Sammanfattning : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. LÄS MER

  4. 4. Advancing isothermal nucleic acid amplification tests : Towards democratization of diagnostics

    Författare :Felix Neumann; Mats Nilsson; Paul Yager; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; MEDICAL AND HEALTH SCIENCES; molecular diagnostics; infectious diseases; point-of-care; digital quantification; fluorescence detection; rolling circle amplification; padlock probes; microfluidic enrichment; biokemi; Biochemistry;

    Sammanfattning : Modern healthcare is the result of scientific advancement across disciplines and has enabled us to understand the rationale behind many diseases and how to treat or cure them; but still a myriad of unanswered questions remains. Especially infectious diseases play an important role in healthcare as they pose a constant threat for global health and well-being. LÄS MER

  5. 5. Detection and Sequencing of Amplified Single Molecules

    Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER