Sökning: "recombinant protein expression"
Visar resultat 21 - 25 av 254 avhandlingar innehållade orden recombinant protein expression.
21. Expression and Mutagenesis studies of Candida antactica lipase B
Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER
22. Mammalian protein expression and characterization tools for next generation biologics
Sammanfattning : Protein therapeutics are increasingly important for modern medicine. Novel recombinant proteins developed today can bind towards their target with high specificity and with low adverse effect. This has enabled the treatment of diseases that for a few years ago were deemed uncurable. LÄS MER
23. Systems Biology of Protein Secretion in Human Cells: Multi-omics Analysis and Modeling of the Protein Secretion Process in Human Cells and its Application
Sammanfattning : Since the emergence of modern biotechnology, the production of recombinant pharmaceutical proteins has been an expanding field with high demand from industry. Pharmaceutical proteins have constituted the majority of top-selling drugs in the pharma industry during recent years. LÄS MER
24. Oxidative stress on mammalian cell cultures during recombinant protein expression
Sammanfattning : When the cell is under stress arising from oxidation, heat, infection, toxic contamination or any other stressful condition, proteins may unfold and expose residues in their structure that under normal physiological conditions are hidden and shielded from chemical reactions.In this licentiate thesis the effects of general oxidative stress on the production of recombinant protein by mammalian cells are considered. LÄS MER
25. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules
Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER