Sökning: "recombinant protein expression"

Visar resultat 16 - 20 av 254 avhandlingar innehållade orden recombinant protein expression.

  1. 16. Vitamin K-dependent protein S - studies of synthesis, structure and function

    Författare :Sofia Somajo; Malmö Klinisk kemi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; C4b-binding protein; coagulation; microparticles; protein C; protein S; structure; tissue factor pathway inhibitor;

    Sammanfattning : This thesis is based on four projects with the overall aim to investigate vitamin K-dependent protein S functions in relation to its structure. The anticoagulant functions of protein S can be divided in three: 1) cofactor for activated protein C (APC), 2) cofactor for tissue factor pathway inhibitor (TFPI) and 3) APC and TFPI-independent anticoagulant functions. LÄS MER

  3. 17. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  4. 18. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r

    Författare :Viveka Dolby; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzymology; Proteiner; enzymologi; Proteins; melanocortin 4 receptor; GPCR; purification; Expression; membrane proteins;

    Sammanfattning : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. LÄS MER

  5. 19. Engineering of Human Fetal Hemoglobin

    Författare :Karin Kettisen; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fetal hemoglobin; protein engineering; recombinant protein production; protein purification; chromatography; oxygen carrier; redox reactions; net surface charge; DNA cleavage;

    Sammanfattning : In blood, the oxygen-transporting protein hemoglobin (Hb) governs the oxygenation of cells and tissues. Naturally, this protein has claimed a place in the center of the research field of artificial oxygen therapeutics. LÄS MER

  7. 20. High-throughput protein analysis using mass spectrometry-based methods

    Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER