Sökning: "Johanna C. Rotticci-Mulder"

Hittade 1 avhandling innehållade orden Johanna C. Rotticci-Mulder.

1. 1. Expression and Mutagenesis studies of Candida antactica lipase B

   Författare :Johanna C. Rotticci-Mulder; KTH; []
   Nyckelord :Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

   Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER