Sökning: "recombinant protein expression"

Visar resultat 11 - 15 av 254 avhandlingar innehållade orden recombinant protein expression.

  1. 11. Membrane and secretory protein production in Escherichia coli

    Författare :Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

    Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER

  3. 12. Viral dUTPases, Recombinant Expression, Purification and Characterization

    Författare :Anna-Carin Bergman; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; nucleocapsid protein; retrovirus; herpesvirus; dUTPase; nucleotide metabolism; enzymology; Proteiner; enzymologi;

    Sammanfattning : The enzyme deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. LÄS MER

  4. 13. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER

  5. 14. Systems Analysis of the Protein Secretory Pathway in Yeast and Human Cells

    Författare :Amir Feizi; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein production; flux balance analysis; biopharmaceutical; secretory pathway: secretome; recombinant protein; genome-scale modeling; systems biology;

    Sammanfattning : The cell was discovered by Robert Hooke in 1665, later in nineteen century the cell theory was developed as all organisms are composed of one or more cells. Since then, many tools and experimental methods have been developed to dissect and characterize the cell components which resulted in established solid scientific fields such as biochemistry, molecular cell biology and genetics. LÄS MER

  7. 15. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER