Sökning: "high-throughput screening"

Visar resultat 16 - 20 av 167 avhandlingar innehållade orden high-throughput screening.

1. 16. Droplet microfluidics for screening and sorting of microbial cell factories

   Författare :Sara Björk; Helene Svahn Andersson; Håkan Jönsson; Christoph Merten; KTH; []
   Nyckelord :Droplet microfluidics; Cell factories; High-throughput screening; Cell culture; Bioteknologi; Biotechnology;

   Sammanfattning : Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. LÄS MER

3. 17. Towards novel antibacterials : Synthesis and identification of natural product inspired inhibitors of Chlamydia trachomatis and development of chemical probes targeting virulence of Pseudomonas aeruginosa

   Författare :Michael Saleeb; Mikael Elofsson; Anna Linusson Jonsson; Andreas Kirschning; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibacterials; antibiotics; small molecules; natural products; benzofuran; dihydrobenzofuran; the type III secretion system; Pseudomonas aeruginosa; Chlamydia trachomatis; phenotypic screening; high-throughput screening; surface plasmon resonance; drug discovery; bacterial toxins; enzyme inhibitors; organisk kemi; Organic Chemistry;

   Sammanfattning : Antibiotic resistance has evolved significantly to become one of the serious threats to public health today. Yet, the pipeline of new antibiotics is drying up and is lagging behind the challenging needs. LÄS MER

4. 18. Intracellular systems for characterization and engineering of proteases and their substrates

   Författare :George Kostallas; Stefan Ståhl; Patrik Samuelson; Matthew DeLisa; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteases; flow cytometry; GFP; CAT; protein engineering; ssrA; ClpXP; tobacco etch virus; TEV; site-specific; high-throughput; selection; proteolysis; libraries; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : Over the years, the view on proteases as relatively non-specific protein degradation enzymes, mainly involved in food digestion and intracellular protein turnover, has shifted and they are now recognized as key regulators of many biological processes that determine the fate of a cell. Besides their biological role, proteases have emerged as important tools in various biotechnical, industrial and medical applications. LÄS MER

5. 19. Explorations of boron-based materials through theoretical simulations

   Författare :Adam Carlsson; Martin Dahlqvist; Johanna Rosén; Jakoah Brgoch; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Density Functional Theory; Thermodynamic Stability; Borides; Crystal Structure Prediction; Cluster Expansion; High-throughput;

   Sammanfattning : This thesis focuses on boron-based materials, notable for their structural complexity and unique combination of ceramic and metallic properties. These properties typically result in materials with high mechanical strength, electrical conductivity, and melting points. LÄS MER

7. 20. Microwell devices for single-cell analyses

   Författare :Sara Lindström; Helene Andersson-Svahn; Mehmet Toner; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; microwell; miniaturization; microfluidics; cell culture; single-cell; clone; imaging; stem cell; cancer; low volume; high-throughput; Molecular biology; Molekylärbiologi;

   Sammanfattning : Powerful tools for detailed cellular studies are emerging, increasing the knowledge ofthe ultimate target of all drugs: the living cell. Today, cells are commonly analyzed inensembles, i.e. thousands of cells per sample, yielding results on the average responseof the cells. LÄS MER