Sökning: "single-cell"

Visar resultat 1 - 5 av 469 avhandlingar innehållade ordet single-cell.

1. 1. Technologies for Single Cell Genome Analysis

   Författare :Erik Borgström; Afshin Ahmadian; Sten Linnarsson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

   Sammanfattning : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. LÄS MER

3. 2. Spatially resolved and single cell transcriptomics

   Författare :Fredrik Salmén; Joakim Lundeberg; Patrik Ståhl; Elaine R. Mardis; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; spatial; transcriptomics; single cell; 3D; RNA-sequencing; Bioteknologi; Biotechnology;

   Sammanfattning : In recent years, massive parallel sequencing has revolutionized the field of biology and has provided us with a vast number of new discoveries in fields such as neurology, developmental biology and cancer research. A significant area is deciphering gene expression patterns, as well as other aspects of transcriptome information, such as the impact of splice variants and mutations on biological functions and disease development. LÄS MER

4. 3. Microwell devices for single-cell analyses

   Författare :Sara Lindström; Helene Andersson-Svahn; Mehmet Toner; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; microwell; miniaturization; microfluidics; cell culture; single-cell; clone; imaging; stem cell; cancer; low volume; high-throughput; Molecular biology; Molekylärbiologi;

   Sammanfattning : Powerful tools for detailed cellular studies are emerging, increasing the knowledge ofthe ultimate target of all drugs: the living cell. Today, cells are commonly analyzed inensembles, i.e. thousands of cells per sample, yielding results on the average responseof the cells. LÄS MER

5. 4. Live Single Cell Imaging and Analysis Using Microfluidic Devices

   Författare :Mohammad Ali Khorshidi; Helene Andersson-Svahn; Håkan Jönsson; Carolina Wählby; Johan Elf; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

   Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

7. 5. Microfluidics and AI for single-cell microbiology

   Författare :Praneeth Karempudi; Johan Elf; Yoav Shechtman; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; Artificial intelligence; Deep learning; Single-cell microbiology; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : Most of the biological sciences deal with understanding the relationships between phenotypes and the underlying molecular mechanisms of organisms. This thesis is an engineering, computational, and experimental exercise in expanding the scope and scale of phenotype-genotype mapping techniques in single-cell microbiology using microscopy, microfluidics, and image processing. LÄS MER