Sökning: "single-cell"

Visar resultat 1 - 5 av 274 avhandlingar innehållade ordet single-cell.

  1. 1. Technologies for Single Cell Genome Analysis

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Erik Borgström; KTH.; [2016]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

    Sammanfattning : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. LÄS MER

  2. 2. Spatially resolved and single cell transcriptomics

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Fredrik Salmén; KTH.; [2017]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; spatial; transcriptomics; single cell; 3D; RNA-sequencing; Biotechnology; Bioteknologi;

    Sammanfattning : In recent years, massive parallel sequencing has revolutionized the field of biology and has provided us with a vast number of new discoveries in fields such as neurology, developmental biology and cancer research. A significant area is deciphering gene expression patterns, as well as other aspects of transcriptome information, such as the impact of splice variants and mutations on biological functions and disease development. LÄS MER

  3. 3. Microwell devices for single-cell analyses

    Detta är en avhandling från Stockholm : KTH

    Författare :Sara Lindström; KTH.; [2009]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; microwell; miniaturization; microfluidics; cell culture; single-cell; clone; imaging; stem cell; cancer; low volume; high-throughput; NATURAL SCIENCES Biology Cell and molecular biology Molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi Molekylärbiologi;

    Sammanfattning : Powerful tools for detailed cellular studies are emerging, increasing the knowledge ofthe ultimate target of all drugs: the living cell. Today, cells are commonly analyzed inensembles, i.e. thousands of cells per sample, yielding results on the average responseof the cells. LÄS MER

  4. 4. Live Single Cell Imaging and Analysis Using Microfluidic Devices

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Mohammad Ali Khorshidi; KTH.; [2013]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

    Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

  5. 5. DNA repair and genome organisation in mammalian cells : studies involving single-cell electrophoresis

    Detta är en avhandling från Stockholm : Stockholm University

    Författare :Stefan Eriksson; Stockholms universitet.; [2000]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical Biology; fysikalisk biologi;

    Sammanfattning : The comet assay (single-cell electrophoresis) is a comparably new method for measurement of DNA strand breakage, primarily in eukaryotic cells. Its main advantage is that it works on the single-cell level, making it possible to detect heterogeneity in DNA damage induction and repair. LÄS MER