Sökning: "high-throughput screening"

Visar resultat 21 - 25 av 167 avhandlingar innehållade orden high-throughput screening.

1. 21. Robustness quantification in yeast: A methodology to study phenotypic, evolutionary, and genomic aspects of microbial robustness

   Författare :Cecilia Trivellin; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; bioproduction; Saccharomyces cerevisiae; adaptive laboratory evolution; High-throughput; fluctuating conditions; perturbations;

   Sammanfattning : Bioprocesses contribute to the shift towards a more sustainable economy. In bioprocesses, valuable chemicals can be generated from renewable resources while, at the same time, reducing carbon emissions. A major hurdle in bringing bio-based products to market is the time and cost involved in designing efficient cell factories. LÄS MER

3. 22. Sensitivity, Noise and Detection of Enzyme Inhibition in Progress Curves

   Författare :Omar Gutierrez Arenas; Helena Danielson; Athel Cornish-Bowden; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Progress curves; limit of detection; Z -factor; primary screening; enzyme inhibition; error structure; sensitivity analysis; High-throughput screning; Biokemi;

   Sammanfattning : Starting with the development of an enzymatic assay, where an enzyme in solution hydrolysed a solid-phase bound peptide, a model for the kinetics of enzyme action was introduced. This model allowed the estimation of kinetic parameters and enzyme activity for a system that has the peculiarity of not being saturable with the substrate, but with the enzyme. LÄS MER

4. 23. Strength in Numbers – Droplet Microfluidics for Multicellular Ensemble Applications

   Författare :Martin Trossbach; Aman Russom; Håkan Jönsson; Nicole Pamme; KTH; []
   Nyckelord :Droplet Microfluidics; High-Throughput Screening; Microtissues; Microreactors; Cell Factories; Cell Culture; Biomaterials; Spheroids; Biotechnology; Bioteknologi;

   Sammanfattning : The work presented in this doctoral thesis explores multicellular, biological, and biotechnological applications for microfluidic droplets, making use of a number of unique features of these miniaturized, highly scalable reaction vessels.Droplet microfluidics specializes in pico- to nanoliter sized aqueous droplets in an immiscible oil phase, borrowing techniques from the field of microfluidics, namely fluid actuation, detection systems and electronic peripherals. LÄS MER

5. 24. New Methods for Chiral Cyanohydrin Synthesis

   Författare :Erica Wingstrand; Christina Moberg; Benjamin List; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; acyl cyanides; asymmetric synthesis; biocatalysis; cyanide; cyanohydrins; dual activation; enzymes; high-throughput screening; Lewis acid; Lewis base; metal catalysis; minor enantiomer recycling; salen; titanium; Chemistry; Kemi;

   Sammanfattning : This thesis deals with method development in asymmetric catalysis and specifically syntheses of enantioenriched O-functionalized cyanohydrins. The first part describes the development of a method for the synthesis of O‑alkoxycarbonylated and O-acylated cyanohydrins. LÄS MER

7. 25. Selective inhibition of acetylcholinesterase 1 from disease-transmitting mosquitoes : design and development of new insecticides for vector control

   Författare :Cecilia Engdahl; Anna Linusson; Fredrik Ekström; Zoran Radić; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; acetylcholinesterase; non-covalent inhibitor; vector control; insecticide; mosquito; vector-borne disease; high throughput screening; rational design; biokemi; Biochemistry; datorlingvistik; computational linguistics;

   Sammanfattning : Acetylcholinesterase (AChE) is an essential enzyme with an evolutionary conserved function: to terminate nerve signaling by rapid hydrolysis of the neurotransmitter acetylcholine. AChE is an important target for insecticides. LÄS MER